Sekine J, Sano K, Hironaka R, Inokuchi T
Second Department of Oral and Maxillofacial Surgery, Nagasaki University, School of Dentistry, Japan.
Okajimas Folia Anat Jpn. 1991 Oct;68(4):219-23. doi: 10.2535/ofaj1936.68.4_219.
To detect S-phase cells in the decalcified paraffin-embedded temporomandibular joint (TMJ) tissues of the rat, bromodeoxyuridine (BrdUrd) immunohistochemistry was performed. Percardiac perfusion of 10% EDTA-2Na Tris buffer accelerated the decalcification. The use of the monoclonal antibody to BrdUrd enabled the visualization of the distribution of S-phase cells. This method is practical in studying cell kinetics and the specific proliferation mode of the TMJ.
为检测大鼠颞下颌关节（TMJ）脱钙石蜡包埋组织中的S期细胞，进行了溴脱氧尿苷（BrdUrd）免疫组织化学检测。经心脏灌注10%乙二胺四乙酸二钠Tris缓冲液可加速脱钙。使用抗BrdUrd单克隆抗体能够观察到S期细胞的分布情况。该方法在研究TMJ的细胞动力学及特定增殖模式方面具有实用性。
