Science. 1963 Jun 21;140(3573):1329-30. doi: 10.1126/science.140.3573.1329.
Lactate dehydrogenase from beef tissues may be resolved electrophoretically into five isozymes each of which is a tetramer. These tetramers can be dissociated into monomers by freezing in 1M sodium chloride. On thawing, reassociation into functional tetramers occurs. On the basis of charge and amino acid composition there are two kinds of monomers. Lactate dehydrogenase-1 contains one kind of monomer and lactate dehydrogenase-5 the other kind. A mixture of equal quantities of these two isozymes, after dissociation and reassociation, leads to the production of all five isozymes in the expected proportions of 1:4:6:4:1.
牛组织中的乳酸脱氢酶经电泳可分离为 5 种同工酶，每种同工酶都是由 4 个亚基组成的四聚体。这些四聚体可通过在 1M 氯化钠中冷冻而解离成单体。解冻后，又重新形成有功能的四聚体。根据电荷和氨基酸组成，有两种类型的单体。乳酸脱氢酶-1 含有一种单体，而乳酸脱氢酶-5 含有另一种单体。两种同工酶等量混合，经解离和重新组合后，可按预期的 1:4:6:4:1 的比例生成全部 5 种同工酶。
