Tian Jin-ying, Ji Teng-fei, Su Ya-lun, Cong Wei-na, Liu Zi-liang, Ye Fei
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1194-8.
To study the hypoglycemic effect of the extract of B. polyandra (SHG).
The diabetic mice were induced by alloxan in ICR mice. The blood glucose concentration was measured by glucose oxidase method. The serum insulin level was determined by 125I-insulin radioimmunoassay kit. The hypoglycemic effect was evaluated by the levels of both fasting and no-fasting blood glucose. The effect on serum insulin level was estimated by the values of the blood insulin and the changes of the blood glucose induced by the glucose intraperitoneal injection. The effect on the glucose absorption was investigated by the oral sucrose or starch tolerance test.
Both of the fasting and no-fasting blood glucose levels were decreased significantly by the treatment of 20 or 30 g raw materials crude drug x kg (-1) SHG orally for 7-10 d in ICR mice or in alloxan diabetic mice. In the oral sucrose tolerance test or oral starch tolerance test, the administration of SHG reduced significantly the peak value of the blood glucose and the area under the blood glucose-time curve (AUC) in normal or alloxan diabetic mice, respectively. These effects of SHG were similar to those of acarbose, a kind of alpha-glucosidase inhibitors. In the oral glucose tolerance test in normal and alloxan diabetic mice, SHG decreased both the blood glucose peak and the AUC induced by the glucose loading. But in the intraperitoneal injection glucose tolerance test the levels of insulin in both SHG and control mice were similar, however, the changes of the blood glucose level after the glucose-loading for 30 min in SHG mice was much lower than that in control mice.
With the treatment of SHG, the fasting and no-fasting blood glucose concentrations were decreased and the glucose tolerance improved significantly in both normal and alloxan diabetic mice, and the inhibition of a-glucosidase might be one of its major mechanisms.
研究多蕊蛇菰提取物（SHG）的降血糖作用。
用四氧嘧啶诱导ICR小鼠成为糖尿病小鼠。采用葡萄糖氧化酶法测定血糖浓度。用125I-胰岛素放射免疫分析试剂盒测定血清胰岛素水平。通过空腹和非空腹血糖水平评估降血糖作用。通过血糖胰岛素值和腹腔注射葡萄糖诱导的血糖变化评估对血清胰岛素水平的影响。通过口服蔗糖或淀粉耐量试验研究对葡萄糖吸收的影响。
在ICR小鼠或四氧嘧啶糖尿病小鼠中，口服20或30 g生药/kg（-1）SHG 7 - 10天，空腹和非空腹血糖水平均显著降低。在口服蔗糖耐量试验或口服淀粉耐量试验中，SHG给药分别显著降低了正常或四氧嘧啶糖尿病小鼠的血糖峰值和血糖-时间曲线下面积（AUC）。SHG的这些作用与α-葡萄糖苷酶抑制剂阿卡波糖的作用相似。在正常和四氧嘧啶糖尿病小鼠的口服葡萄糖耐量试验中，SHG降低了葡萄糖负荷诱导的血糖峰值和AUC。但在腹腔注射葡萄糖耐量试验中，SHG小鼠和对照小鼠的胰岛素水平相似，然而，SHG小鼠在葡萄糖负荷30分钟后的血糖水平变化远低于对照小鼠。
SHG治疗后，正常和四氧嘧啶糖尿病小鼠的空腹和非空腹血糖浓度均降低，葡萄糖耐量显著改善，抑制α-葡萄糖苷酶可能是其主要机制之一。
