Xiao Yan-ling, Lu Fu-er, Xu Li-jun, Leng San-hua, Wang Kai-fu
Institute of Integrative Traditional & Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
Zhongguo Zhong Yao Za Zhi. 2005 Nov;30(22):1767-70.
To investigate the effects of Huanglian Jiedu (HLJD) decoction on vascular endothelial function in type 2 diabetic rats and explore the prophylactic and therapeutic significance and pharmacological mechanism of HLJD decoction in type 2 diabetic angiopathic complication.
The murine type 2 diabetes models were induced by the intravenous injection of a small dose of streptozotocin plus high fat and high caloric laboratory chow. Then modeled diabetic animals were divided into model group, HLJD group, and aspirin group. Normal ratsfed with routine chow were designated as normal group. The oral glucose tolerance test (OGTT) were performed in all animals, 9 weeks after treatment, the changes of murine body weights and levels of fasting blood glucose (FBG), serum total cholesterol (TC), triglycerides (TG), high density lipoprotein-cholesterol(HDL-C), fasting serum insulin (FINS), serum nitric oxide, plasma endothelin, angiotensin II and von Willebrand Factor (vWF) were determined 10 weeks after treatment.
Compared with model group, the result of OGTT of HLJD group was improved. The levels of the body weights, TC, TG and ET in HLJD group weredecreased (P < 0.05). The levels of FBG,INS, AngII and vWFwere significantly decreased (P < 0.01), and the levels of HDL-C and NO were obviously increased (P < 0.05), as compared with those in model group. Furthermore. The levels of FBG was lower in HLJD group than in aspirin group (P < 0.05), and the improvement of TG, HDL-C,NO, AngII, vWF levels in HLJD group was more greatly than that in aspirin group, but there was not significant difference between two groups (P > 0.05).
It is suggested by the present results that HLJD decoction could protect vascular endothelium from early damage in type 2 diabetes. The protective effects of HLJD on endothelium might be related to its ability of reducing the blood glucose, adjusting plasma lipids profiles, improving insulin resistance, antagonizing inflammatory mediators and inducing endothelium-dependent vascular relaxation.
探讨黄连解毒汤对2型糖尿病大鼠血管内皮功能的影响，探讨黄连解毒汤对2型糖尿病血管病变并发症的防治意义及药理机制。
采用小剂量链脲佐菌素腹腔注射加高脂高糖饲料喂养诱导小鼠2型糖尿病模型。将造模成功的糖尿病动物分为模型组、黄连解毒汤组和阿司匹林组。以常规饲料喂养的正常大鼠作为正常组。对所有动物进行口服葡萄糖耐量试验（OGTT），治疗9周后，测定小鼠体重变化，治疗10周后测定空腹血糖（FBG）、血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、空腹血清胰岛素（FINS）、血清一氧化氮、血浆内皮素、血管紧张素Ⅱ及血管性血友病因子（vWF）水平。
与模型组比较，黄连解毒汤组OGTT结果改善。黄连解毒汤组体重、TC、TG及ET水平降低（P<0.05）。与模型组比较，黄连解毒汤组FBG、INS、AngⅡ及vWF水平显著降低（P<0.01），HDL-C及NO水平明显升高（P<0.05）。此外，黄连解毒汤组FBG水平低于阿司匹林组（P<0.05），黄连解毒汤组TG、HDL-C、NO、AngⅡ、vWF水平改善程度大于阿司匹林组，但两组比较差异无统计学意义（P>0.05）。
本研究结果提示，黄连解毒汤可保护2型糖尿病早期血管内皮损伤。黄连解毒汤对内皮的保护作用可能与其降低血糖、调节血脂、改善胰岛素抵抗、拮抗炎症介质及诱导内皮依赖性血管舒张的能力有关。
