Ji Kang-ting, Zhang Huai-qin, Tang Ji-fei, Li Hai-ying
Department of Cardiology, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1214-7.
To investigate the effects of Danshen on number and activity of endothelial progenitor cells (EPCs) of patients with Hypercholesterolemia.
24 patients with Hypercholesterolemia were randomLy divided into 2 groups: control group (n = 12), and treatment group (n = 12, receiving Composite Denshen Pilulae, 10# tid for 2 weeks). after 2 weeks, 20 mL peripheral blood was obtained from each patient, Mononuclear fraction of human peripheral blood was obtained by density gradient centrifugation, plated on fibronectin coated culture dishes. The cells were identified by immunohistochemistry and flow cytometry and tested the ability to intake ac-LDL. Cell clusters were viewed with an inverted microscope, fluorescence-activated cell sorting (FACS) analysis of PE-CD34 and FITC-AC133 was performed to detect number of EPCs, EPC proliferation and migration were assayed with MTT assay, modified Boyden chamber assay. EPCs adhesion ability assay was performed by replating cells on fibronectin-coated dishes, and then counting adherent cells.
Numbers of EPCs (10(3) cells per 1 mL peripheral blood) of treatment group was higher than control group (7.20 +/- 1.29 vs 6.88 +/- 1.00). Compared with group control, numbers of clusters (per 40 power microscopic field), adhesive EPCs (per 400 power microscopic field) and migratory EPCs (per 200 power microscopic field) of treatment group were significantly increased (4.47 +/- 0.94 vs 3.38 +/- 0.57, P <0.01, 11.81 +/- 2.29 vs 10.03 +/- 1.32, P <0.05 and 15.75 +/- 2.27 vs 11.95 +/- 1.28, P <0.01, respectively), while OD vallue of treatment group were significantly increased too (0.27 +/- 0.04 vs 0. 20 +/- 0.03, P < 0.01).
Danshen can significantly enhance EPCs functional activity of patients with Hypercholesterolemia.
探讨丹参对高胆固醇血症患者内皮祖细胞（EPCs）数量及活性的影响。
将24例高胆固醇血症患者随机分为2组：对照组（n = 12）和治疗组（n = 12，服用复方丹参丸，每次10丸，每日3次，共2周）。2周后，采集每位患者20 mL外周血，通过密度梯度离心法获取人外周血单个核细胞，接种于纤连蛋白包被的培养皿上。采用免疫组织化学和流式细胞术对细胞进行鉴定，并检测其摄取乙酰化低密度脂蛋白（ac-LDL）的能力。用倒置显微镜观察细胞集落，进行PE-CD34和FITC-AC133的荧光激活细胞分选（FACS）分析以检测EPCs数量，采用MTT法、改良Boyden小室法检测EPCs的增殖和迁移能力。通过将细胞重新接种于纤连蛋白包被的培养皿上，然后计数贴壁细胞来进行EPCs黏附能力检测。
治疗组EPCs数量（每1 mL外周血中的10³个细胞）高于对照组（7.20±1.29对6.88±1.00）。与对照组相比，治疗组的集落数量（每40倍显微镜视野）、黏附EPCs数量（每400倍显微镜视野）和迁移EPCs数量（每200倍显微镜视野）均显著增加（分别为4.47±0.94对3.38±0.57，P<0.01；11.81±2.29对10.03±1.32，P<0.05；15.75±2.27对11.95±1.28，P<0.01），同时治疗组的OD值也显著增加（0.27±0.04对0.20±0.03，P<0.01）。
丹参可显著增强高胆固醇血症患者EPCs的功能活性。
