Litskevich L A
Probl Endokrinol (Mosk). 1991 Sep-Oct;37(5):47-50.
A study was made of metabolic derangement of exogenous cortisol in isolated perfused liver of irradiated rats. The animals were exposed to x-radiation at doses of 2, 4, 8, 10 and 12 Gy. A 72 h irradiation was followed by decapitation and perfusion of the liver with cortisol. Cortisol and its metabolites in a perfusion medium were determined by thin-layer chromatography. Irradiation at the doses of 2 and 4 Gy resulted in no noticeable changes of steroid-metabolizing liver function. At the lethal doses of 8, 10 and 12 Gy cortisol absorption by the liver was gradually decreased and accompanied by accumulation in the perfusion medium of excessive amounts (as compared to control) of tetrahydrocortisone, cortisone, and unidentified metabolites that may be associated with disorder of their conversion at subsequent stages of metabolism. A method of selective extraction of varied polarity fractions of hydrocortisone metabolites from a perfusate has also shown that irradiation inhibits transformation of steroids into more polar compounds.
对受辐照大鼠离体灌注肝脏中外源皮质醇的代谢紊乱进行了一项研究。将动物暴露于2、4、8、10和12 Gy剂量的X射线辐射下。在72小时的辐照后，将动物断头并使用皮质醇对肝脏进行灌注。通过薄层色谱法测定灌注介质中的皮质醇及其代谢产物。2和4 Gy剂量的辐照未导致类固醇代谢肝功能出现明显变化。在8、10和12 Gy的致死剂量下，肝脏对皮质醇的吸收逐渐减少，并伴随着灌注介质中（与对照相比）过量的四氢皮质醇、可的松和可能与其在后续代谢阶段转化紊乱有关的未鉴定代谢产物的积累。一种从灌注液中选择性提取氢化可的松代谢产物不同极性组分的方法也表明，辐照会抑制类固醇转化为极性更强的化合物。
