Ben Othmen Leila, Mechri Anwar, Fendri Chiraz, Bost Muriel, Chazot Guy, Gaha Lotfi, Kerkeni Abdelhamid
Research Laboratory Trace Elements, Free Radicals and Antioxidants, Biophysics Department, Faculty of Medicine, 5000 Monastir, Tunisia.
Prog Neuropsychopharmacol Biol Psychiatry. 2008 Jan 1;32(1):155-9. doi: 10.1016/j.pnpbp.2007.08.003. Epub 2007 Aug 14.
To determine Red Blood Cell (RBC) antioxidant enzyme activities and plasma Thiobarbituric Acid Reactive Substances (TBARS) in clinically stable patients with schizophrenia and their unaffected siblings.
A case-control study carried out on three groups: 60 schizophrenic patients treated with neuroleptics, 33 of their unaffected siblings and 30 healthy controls with no family psychiatric history. Biological markers were measured on fasting patients after a period of tobacco abstinence: RBC antioxidant enzyme activities - superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) - by spectrophotometry and plasma levels of TBARS by spectrofluorimetry.
RBC SOD and CAT activities were significantly lower in schizophrenic patients and their unaffected siblings compared to the control group (P<0.001). Schizophrenic patients also had significantly lower RBC GSH-Px activity than controls (P=0.03), whereas their unaffected siblings had significantly higher RBC GSH-Px activity than controls (P=0.04). Plasma TBARS were higher in schizophrenic patients than their unaffected siblings: 2.1+/-0.8 micromol/l vs. 1.7+/-0.6 micromol/l (P=0.06).
Our results showed a decrease in antioxidant enzyme activities and an increase in lipid peroxidation confirming the existence of oxidative stress in schizophrenic patients treated with neuroleptics. Additionally, this suggests that the increase in GSH-Px activity in unaffected siblings would be a protective mechanism against oxidative stress and damage. Other studies are necessary to confirm these findings.
测定临床病情稳定的精神分裂症患者及其未患病同胞的红细胞抗氧化酶活性和血浆硫代巴比妥酸反应性物质（TBARS）水平。
开展一项病例对照研究，分为三组：60例接受抗精神病药物治疗的精神分裂症患者、33例其未患病的同胞以及30名无家族精神病史的健康对照者。在患者戒烟一段时间后，于空腹状态下测量生物学标志物：通过分光光度法测定红细胞抗氧化酶活性——超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT），通过荧光分光光度法测定血浆TBARS水平。
与对照组相比，精神分裂症患者及其未患病同胞的红细胞SOD和CAT活性显著降低（P<0.001）。精神分裂症患者的红细胞GSH-Px活性也显著低于对照组（P=0.03），而其未患病同胞的红细胞GSH-Px活性显著高于对照组（P=0.04）。精神分裂症患者的血浆TBARS水平高于其未患病同胞：分别为2.1±0.8微摩尔/升和1.7±0.6微摩尔/升（P=0.06）。
我们的结果显示抗氧化酶活性降低以及脂质过氧化增加，证实了接受抗精神病药物治疗的精神分裂症患者存在氧化应激。此外，这表明未患病同胞中GSH-Px活性的增加可能是一种针对氧化应激和损伤的保护机制。需要其他研究来证实这些发现。
