Katoh-Semba Ritsuko, Wakako Rie, Komori Taku, Shigemi Hiroko, Miyazaki Noriko, Ito Hironori, Kumagai Toshiyuki, Tsuzuki Masako, Shigemi Kenji, Yoshida Futoshi, Nakayama Atsuo
Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan.
Int J Dev Neurosci. 2007 Oct;25(6):367-72. doi: 10.1016/j.ijdevneu.2007.07.002. Epub 2007 Aug 1.
Accumulating evidence suggests the possible association between the concentrations of serum brain-derived neurotrophic factor (BDNF) and psychiatric disease with impaired brain development. Yet the reasons remain unclear. We therefore investigated the characteristics of serum BDNF as well as its age-related changes in healthy controls in comparison to autism cases. BDNF was gradually released from platelets at 4 degrees C, reached a maximal concentration after around 24 h, and remained stable until 42 h. At room temperature, BDNF was found to be immediately degraded. Circadian changes, but not seasonal changes, were found in serum levels of BDNF existing as the mature form with a molecular mass of 14 kDa. In healthy controls, the serum BDNF concentration increased over the first several years, then slightly decreased after reaching the adult level. There were no sex differences between males and females. In the autism cases, mean levels were significantly lower in children 0-9 years old compared to teenagers or adults, or to age-matched healthy controls, indicating a delayed BDNF increase with development. In a separate study of adult rats, a circadian change in serum BDNF was found to be similar to that in the cortex, indicating a possible association with cortical functions.
越来越多的证据表明，血清脑源性神经营养因子（BDNF）浓度与脑发育受损的精神疾病之间可能存在关联。然而，其原因尚不清楚。因此，我们研究了血清BDNF的特征及其在健康对照与自闭症病例中的年龄相关变化。BDNF在4℃下从血小板中逐渐释放，约24小时后达到最大浓度，并在42小时内保持稳定。在室温下，BDNF被发现会立即降解。血清中以分子量为14 kDa的成熟形式存在的BDNF水平存在昼夜变化，但无季节性变化。在健康对照中，血清BDNF浓度在最初几年升高，达到成人水平后略有下降。男性和女性之间没有性别差异。在自闭症病例中，0至9岁儿童的平均水平显著低于青少年或成年人，或与年龄匹配的健康对照，表明BDNF随发育的增加延迟。在另一项对成年大鼠的研究中，发现血清BDNF的昼夜变化与皮质中的变化相似，表明可能与皮质功能有关。
