Chen F, Feng X, Wu W, Ouyang H, Gao Z, Cheng X, Hou R, Mao T
Xi'da-RegeMed Lab of Tissue Engineering, Faculty of Life Science, Northwest University, Xi'an 710069, PR China.
Int J Oral Maxillofac Surg. 2007 Sep;36(9):822-7. doi: 10.1016/j.ijom.2007.06.019. Epub 2007 Sep 4.
The size and specific shape of bone grafts are important for jaw reconstruction. In this experiment, segmental bone grafts were engineered in a predetermined shape via seeding osteoblast precursor cells into titanium mesh-coral composite scaffolds. Titanium meshes were moulded into the shape of a column with length 12 mm and diameter 8mm. The column was filled with natural coral granules and the complex acted as a cell-seeding scaffold. About 4 x 10(7) osteoblast precursor cells in 200 microl cell-culture medium were seeded into each of six scaffolds and incubated in vitro for 2 days. Then, the composites were implanted subcutaneously into the backs of nude mice and incubated in vivo. Two months after implantation, the animals were killed and new bone formed in the scaffolds was investigated by gross inspection, X-ray examination, histological observation and mechanical testing. The results showed that newly formed tissue was red and presented the gross appearance of bone, and kept the original shape of the column. Titanium mesh was situated on the surface of the bone graft. An X-ray blocking shadow was observed in and around the titanium scaffolds; most of the coral granules had been absorbed. Histological observation demonstrated a large amount of new bone formed and integrated well with titanium mesh. Mechanical testing showed that new bone improved the mechanical property of the graft significantly. In conclusion, a titanium mesh-coral composite scaffold with osteoblast precursor cells is an efficient means to engineer segmental bone, possessing the desired shape and mechanical strength.
骨移植材料的大小和特定形状对于颌骨重建至关重要。在本实验中，通过将成骨细胞前体细胞接种到钛网-珊瑚复合支架中，构建出预定形状的节段性骨移植材料。将钛网模制成长度为12毫米、直径为8毫米的柱状。该柱状物填充天然珊瑚颗粒，该复合体作为细胞接种支架。将200微升细胞培养基中约4×10⁷个成骨细胞前体细胞接种到六个支架中的每一个中，并在体外培养2天。然后，将复合材料皮下植入裸鼠背部并在体内培养。植入两个月后，处死动物，通过大体检查、X线检查、组织学观察和力学测试对支架中形成的新骨进行研究。结果显示，新形成的组织呈红色，具有骨的大体外观，并保持柱状的原始形状。钛网位于骨移植材料的表面。在钛支架内部及周围观察到X线阻射阴影；大部分珊瑚颗粒已被吸收。组织学观察显示形成了大量新骨，且与钛网整合良好。力学测试表明，新骨显著改善了移植材料的力学性能。总之，带有成骨细胞前体细胞的钛网-珊瑚复合支架是构建节段性骨的有效方法，具有所需的形状和力学强度。
