Chen Fulin, Chen Shujun, Tao Kai, Feng Xue, Liu Yanpu, Lei Delin, Mao Tianqiu
Department of Oral Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Kang Fu Road 7, Xi'an 710032, PR China.
Br J Oral Maxillofac Surg. 2004 Dec;42(6):532-7. doi: 10.1016/j.bjoms.2004.08.007.
To find out if it was possible to prefabricate bone graft in the shape of a human mandibular ramus that possessed a pedicle that carried blood. The pore size of the natural coral was about 200 microm with a porosity of about 36%. The natural coral was made into the shape of a human mandibular ramus. Marrow-derived osteoblasts were seeded into porous natural coral scaffolds in a density of 2 x 10(8)/mL in 300 microL cell suspension. After two days of in vitro incubation, five cell-coral complexes were implanted into cell donor rabbits under the inferior epigastric blood vessels to prefabricate a vascularised bone graft of specific shape. Two months later the bone formation was observed by gross inspection and histological examination. Two months after operation, a well-vascularised bone graft in the shape of the initial coral scaffold and with a blood-carrying pedicle had been regenerated successfully. Osteogenesis followed the pattern of endochondral bone formation. New bone could be seen on the surface and in the pores of coral on histological examination. We have shown that it is possible to fabricate vascularised bone graft in a predetermined shape using tissue engineering. This kind of bone graft may have future clinical application.
为了探究是否有可能预制出具有带血蒂的人下颌支形状的骨移植体。天然珊瑚的孔径约为200微米，孔隙率约为36%。将天然珊瑚制成人下颌支的形状。将骨髓来源的成骨细胞以2×10⁸/mL的密度接种到300微升细胞悬液中的多孔天然珊瑚支架上。体外培养两天后，将五个细胞 - 珊瑚复合物植入细胞供体兔的腹下血管下方，以预制特定形状的带血管化骨移植体。两个月后，通过大体检查和组织学检查观察骨形成情况。术后两个月，成功再生出了具有初始珊瑚支架形状且带有带血蒂的良好血管化骨移植体。成骨遵循软骨内成骨模式。组织学检查可见珊瑚表面和孔隙中有新骨形成。我们已经表明，使用组织工程技术预制预定形状的带血管化骨移植体是可行的。这种骨移植体可能具有未来的临床应用价值。
