Nair Manitha B, Suresh Babu S, Varma H K, John Annie
Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum 695 012, India.
Acta Biomater. 2008 Jan;4(1):173-81. doi: 10.1016/j.actbio.2007.07.004. Epub 2007 Jul 27.
Scaffolds which encourage the incorporation of a cell source for tissue engineering applications are critical determinants for clinical defects. Over the years, a number of biomaterials have emerged for cell support and growth, but only a few have demonstrated clinical efficacy. We therefore investigated an in-house-developed silica-based bioactive ceramic for its ability to support and sustain the growth of bone marrow-derived mesenchymal stem cells (BMSCs) in vitro. For this, MSCs aspirated from goat bone marrow were isolated and culture expanded on a novel triphasic ceramic composite coated hydroxyapatite (HASi) scaffold comprising hydroxyapatite, tricalcium phosphate and calcium silicate. The viability of cells that harbored on and within the material was ensured through fluorescence-activated cell sorting and confocal laser scanning microscope and for their anchorage sites by scanning electron microscopy. Interestingly, over the days in culture, cell-cell interactions gradually morphed into woven cell-sheets that spanned across the surface of the HASi, forming a canopy. To conclude, we have attempted to carry out the preliminary cytocompatibility studies of this novel ceramic to establish its appropriateness for bone tissue engineering application which is an important criterion in orthopaedic transplantation and regenerative surgery.
对于组织工程应用而言，能够促进细胞源整合的支架是临床缺损的关键决定因素。多年来，出现了多种用于细胞支持和生长的生物材料，但只有少数几种显示出临床疗效。因此，我们研究了一种内部开发的基于二氧化硅的生物活性陶瓷在体外支持和维持骨髓间充质干细胞（BMSC）生长的能力。为此，从山羊骨髓中提取的间充质干细胞被分离出来，并在一种新型的三相陶瓷复合材料涂层羟基磷灰石（HASi）支架上进行培养扩增，该支架由羟基磷灰石、磷酸三钙和硅酸钙组成。通过荧光激活细胞分选和共聚焦激光扫描显微镜确保了附着在材料上和材料内部的细胞的活力，并通过扫描电子显微镜观察了它们的锚定部位。有趣的是，在培养的几天里，细胞间相互作用逐渐演变成横跨HASi表面的编织状细胞片，形成一个覆盖层。总之，我们试图对这种新型陶瓷进行初步的细胞相容性研究，以确定其在骨组织工程应用中的适用性，这是骨科移植和再生手术中的一个重要标准。
