Kuemmel D F, Chapman L R
The Procter & Gamble Co., Miami Valley Laboratories, 45239, Cincinnati, Ohio.
Lipids. 1968 Jul;3(4):313-6. doi: 10.1007/BF02530930.
The monoenoic methyl esters from numerous fats and oils which contained appreciablecis-9-hexadecenoic acid (cis-9-16ratio1) were isolated by liquid-solid chromatography on silver nitrate-silica gel. Analysis of the monoenes by packed and capillary column gas-liquid chromatography showed that significant amounts ofcis-11-octadecenoic acid (cis-11-18ratio1) were present in all samples. The amount ofcis-11-18ratio1 found in the monoenoic methyl esters increased proportionally to logarithmic increases in thecis-9-16ratio1 level. Most analyses reported in the literature also show this proportionality. This mathematical relationship suggests that chain elongation ofcis-9-16ratio1 tocis-11-18ratio1 is a biosynthetic pathway operative in a wide variety of species.
通过在硝酸银-硅胶上进行液固色谱法，从众多含有可观顺式-9-十六碳烯酸（顺式-9-16比例≥1）的油脂中分离出单烯酸甲酯。通过填充柱和毛细管柱气液色谱法对单烯进行分析表明，所有样品中均存在大量顺式-11-十八碳烯酸（顺式-11-18比例≥1）。在单烯酸甲酯中发现的顺式-11-18比例随着顺式-9-16比例水平的对数增加而成比例增加。文献中报道的大多数分析也表明了这种比例关系。这种数学关系表明，顺式-9-16比例到顺式-11-18比例的链延长是在多种物种中起作用的生物合成途径。
