白细胞中的鞘脂代谢。I. 完整人类白细胞将（14）C-葡萄糖和（14）C-半乳糖掺入鞘糖脂的过程。

Sphingolipid metabolism in leucocytes. I. Incorporation of(14)C-glucose and (14)C-galactose into glycosphingolipids by intact human leucocytes.

作者信息

Kampine J P, Martensson E, Yankee R A, Kanfer J N

机构信息

Laboratory of Neurochemistry, National Institute of Neurological Diseases and Blindness, National Institutes of Health, 20014, Bethesda, Maryland.

出版信息

Lipids. 1968 Mar;3(2):151-6. doi: 10.1007/BF02531733.

DOI:10.1007/BF02531733

PMID:17805904

Abstract

Glucosyl ceramide and lactosyl ceramide have been isolated from intact human leucocytes. Incubation of intact white blood cells with either(14)C-glucose or(14)C-galactose resulted in the incorporation of these tracers into the glycosphingolipids. The products were extracted by conventional procedures and purified by combined silicic acid column and thin-layer chromatography. The bulk of the radioactivity was found in the monohexoside and dihexoside ceramide fractions.Acid hydrolysis yielded glucose as the principal carbohydrate of the monohexoside ceramide, regardless of the sugar precursor employed. In the dihexoside ceramide fraction, galactose was liberated as the major sugar component. The specific activities of the lactosyl ceramide was found to be greater than that of the corresponding glucosyl ceramide.

摘要

葡萄糖神经酰胺和乳糖神经酰胺已从完整的人白细胞中分离出来。将完整的白细胞与（14）C-葡萄糖或（14）C-半乳糖一起孵育，会导致这些示踪剂掺入糖鞘脂中。产物通过常规方法提取，并通过硅胶柱和薄层色谱法联合纯化。大部分放射性存在于单己糖苷和二己糖苷神经酰胺馏分中。无论使用何种糖前体，酸水解产生的葡萄糖都是单己糖苷神经酰胺的主要碳水化合物。在二己糖苷神经酰胺馏分中，半乳糖作为主要糖类成分被释放出来。发现乳糖神经酰胺的比活性高于相应的葡萄糖神经酰胺。