鸡红细胞中性鞘脂的特性分析。

Characterization of neutral sphingolipids from chicken erythrocytes.

作者信息

Shiraishi T, Uda Y

出版信息

J Lipid Res. 1985 Jul;26(7):860-6.

Abstract

The neutral sphingolipids from chicken erythrocytes were characterized. The total concentration of neutral sphingolipids was found to be 480 nmol/g of dry stroma. They were isolated and purified by droplet counter-current chromatography, Iatrobeads column chromatography, and preparative thin-layer chromatography. The major neutral sphingolipids were free ceramide, ceramide monohexoside, ceramide dihexoside, and ceramide pentahexoside, which represented 43%, 23.5%, 10.0%, and 3.6% of the long chain bases, respectively. Thus, free ceramide was the most abundant neutral sphingolipid in chicken erythrocytes. Ceramide monohexoside was composed of more galactosylceramide than glucosylceramide. Galabiosylceramide was found in the ceramide dihexoside fraction together with lactosylceramide. Ceramide pentahexoside was a Forssman glycolipid. There were two groups of neutral sphingolipids; one had mainly C16 fatty acid and the other had C22 and C24 fatty acids. In both groups sphingosine (d18:1) was predominant as a long chain base. 2-Hydroxy-C16 fatty acid was a major component of one of the ceramide monohexosides.

摘要

对鸡红细胞中的中性鞘脂进行了表征。发现中性鞘脂的总浓度为480 nmol/g干基质。通过液滴逆流色谱法、Iatrobeads柱色谱法和制备型薄层色谱法对它们进行了分离和纯化。主要的中性鞘脂是游离神经酰胺、神经酰胺单己糖苷、神经酰胺二己糖苷和神经酰胺五己糖苷，它们分别占长链碱基的43%、23.5%、10.0%和3.6%。因此，游离神经酰胺是鸡红细胞中最丰富的中性鞘脂。神经酰胺单己糖苷中半乳糖神经酰胺的含量多于葡萄糖神经酰胺。在神经酰胺二己糖苷部分中发现了半乳糖二糖神经酰胺和乳糖神经酰胺。神经酰胺五己糖苷是福斯曼糖脂。有两组中性鞘脂；一组主要含有C16脂肪酸，另一组含有C22和C24脂肪酸。在两组中，鞘氨醇（d18:1）作为长链碱基占主导地位。2-羟基-C16脂肪酸是其中一种神经酰胺单己糖苷的主要成分。