Schwerin M, Blottner S, Thomsen P D, Roschlau D, Brockmann G
Department of Molecular Genetics, Research Center of Animal Production Dummerstorf-Rostock, Federal Republic of Germany.
Mol Reprod Dev. 1991 Sep;30(1):39-43. doi: 10.1002/mrd.1080300106.
An easy assay for quantification of Y chromosome-bearing sperm (Y-sperm) is needed, especially to monitor sperm separation techniques. In the present study a tritiated bovine male-specific DNA fragment was tested for identification of Y-sperm by in situ hybridization. A protocol for in situ hybridization to bovine sperm was developed and used to study the proportion of Y-sperm of 12 bulls. The usefulness of the method in optimization of sperm separation procedures is illustrated through analysis of fractions of sperm separated by Percoll density gradient centrifugation.
需要一种简便的方法来定量含Y染色体的精子（Y精子），尤其是用于监测精子分离技术。在本研究中，对一个氚标记的牛雄性特异性DNA片段进行了测试，以通过原位杂交鉴定Y精子。制定了一种用于牛精子原位杂交的方案，并用于研究12头公牛的Y精子比例。通过分析经Percoll密度梯度离心分离的精子组分，说明了该方法在优化精子分离程序中的实用性。
