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无细胞蛋白质合成中指导合成的人工多核苷酸的命运 II. 与核糖体的结合。

Fate of a Synthetic Polynucleotide Directing Cell-Free Protein Synthesis II. Association with Ribosomes.

出版信息

Science. 1962 Nov 16;138(3542):813-7. doi: 10.1126/science.138.3542.813.

DOI:10.1126/science.138.3542.813
PMID:17820995
Abstract

Tritiated-poly U, when added to cell-free extracts of Escherichia coli, became associated with ribosomes. This association occurred at 3 degrees C and did not require high-energy phosphate compounds. Small amounts of tritiated polyuridylic acid produced polydisperse ribosomal aggregates with sedimentation constants of approximately 100 to 130. C(14)-phenylalanine initially was incorporated into protein only on these particles, which suggests that they are the sites of polyphenylalanine synthesis.

摘要

当氚标记的多聚 U 被添加到大肠杆菌的无细胞提取物中时，它与核糖体结合。这种结合发生在 3°C，不需要高能磷酸化合物。少量的氚标记多尿嘧啶核苷酸产生多分散的核糖体聚集体，沉降常数约为 100 到 130。最初，C(14)-苯丙氨酸仅在这些颗粒上被掺入蛋白质，这表明它们是多苯丙氨酸合成的部位。

相似文献

1
Fate of a Synthetic Polynucleotide Directing Cell-Free Protein Synthesis II. Association with Ribosomes.无细胞蛋白质合成中指导合成的人工多核苷酸的命运 II. 与核糖体的结合。
Science. 1962 Nov 16;138(3542):813-7. doi: 10.1126/science.138.3542.813.
2
Fate of a Synthetic Polynucleotide Directing Cell-Free Protein Synthesis I. Characteristics of Degradation.无细胞蛋白质合成中指导合成的人工多核苷酸的命运 I. 降解特性。
Science. 1962 Nov 16;138(3542):810-3. doi: 10.1126/science.138.3542.810.
3
Reassembly of functionally active 50S ribosomal particles from proteins and RNAs of Escherichia coli. Dependency of 50S ribosomal reassembly on 30S subunits.利用大肠杆菌的蛋白质和RNA重新组装功能活性50S核糖体颗粒。50S核糖体重新组装对30S亚基的依赖性。
J Biochem. 1975 Jan 1;77(1?):43-54.
4
Effect of preincubation in the cold on poly(U)-dependent polyphenylalanine synthesis in a cell-free rat liver system.低温预孵育对无细胞大鼠肝脏系统中聚（U）依赖性聚苯丙氨酸合成的影响。
Acta Biol Med Ger. 1980;39(1):41-6.
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Study of the mechanism of translocation in ribosomes. 1. Polyphenylalanine synthesis in Escherichia coli ribosomes without participation of guanosine-5'-triphosphate and protein translation factors.核糖体转位机制的研究。1. 大肠杆菌核糖体中多聚苯丙氨酸的合成，无需鸟苷-5'-三磷酸和蛋白质翻译因子的参与。
Mol Biol. 1971 Nov-Dec;5(6):710-7.
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[Isolation of ribosomal subparticles from human placenta containing intact rRNA and determination of the functional activity of the 80S ribosome].[从人胎盘中分离出含有完整核糖体RNA的核糖体亚颗粒并测定80S核糖体的功能活性]
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[Ribosomal synthesis of polyleucine on a polyuridylic acid matrix in a cell-free translation system].[在无细胞翻译系统中聚尿苷酸模板上多聚亮氨酸的核糖体合成]
Dokl Akad Nauk SSSR. 1980;254(3):766-8.
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Effect of food deprivation and hypophysectomy on in vitro protein synthesis by membrain-bound and free hepatic ribosomes.食物剥夺和垂体切除对肝膜结合核糖体和游离核糖体体外蛋白质合成的影响。
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Protein synthesis in cell-free systems from totally ischemic rat myocardium.来自完全缺血大鼠心肌的无细胞系统中的蛋白质合成。
Recent Adv Stud Cardiac Struct Metab. 1975;7:341-7.
10
Coupling of rRNA transcription and ribosomal assembly in vivo. Formation of active ribosomal subunits in Escherichia coli requires transcription of rRNA genes by host RNA polymerase which cannot be replaced by bacteriophage T7 RNA polymerase.体内rRNA转录与核糖体组装的偶联。在大肠杆菌中形成活性核糖体亚基需要宿主RNA聚合酶转录rRNA基因，而噬菌体T7 RNA聚合酶无法替代宿主RNA聚合酶。
J Mol Biol. 1993 Jun 5;231(3):581-93. doi: 10.1006/jmbi.1993.1311.

引用本文的文献

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Cell-free synthetic biology for natural product biosynthesis and discovery.用于天然产物生物合成与发现的无细胞合成生物学
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Polyribosome Metabolism in II. Formation and Lifetime of Messenger RNA Molecules, Ribosomal Subunit Couples and Polyribosomes.II. 信使RNA分子、核糖体亚基偶联物和多核糖体的形成与寿命中的多核糖体代谢
J Mol Biol. 1967 Nov 14;29(3):395-418. doi: 10.1016/0022-2836(67)90107-6. Epub 2004 Oct 27.
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Ribosome activation and polysome formation in vitro: requirement for ATP.
体外核糖体激活与多核糖体形成：对ATP的需求
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1770-7. doi: 10.1073/pnas.56.6.1770.
4
SPECIFIC BINDING OF SRNA WITH THE TEMPLATE-RIBOSOME COMPLEX.小分子RNA与模板-核糖体复合物的特异性结合。
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1541-7. doi: 10.1073/pnas.52.6.1541.
5
DANON D: POLYRIBOSOMES AND PROTEIN SYNTHESIS DURING RETICULOCYTE MATURATION IN VITRO.达农D：体外网织红细胞成熟过程中的多核糖体与蛋白质合成
Proc Natl Acad Sci U S A. 1963 Aug;50(2):336-42. doi: 10.1073/pnas.50.2.336.
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Studies on the nature of polysomes.多核糖体性质的研究。
Biochem J. 1964 Aug;92(2):289-99. doi: 10.1042/bj0920289.
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A possible mechanism for initiation of protein synthesis.蛋白质合成起始的一种可能机制。
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