从泌乳和非泌乳牛乳腺制备的核糖体的差异。
Differences in the ribosomes prepared from lactating and non-lactating bovine mammary gland.
作者信息
Herrington M D, Hawtrey A O
出版信息
Biochem J. 1971 Jan;121(2):279-85. doi: 10.1042/bj1210279.
Abstract
- Ribosomes prepared from bovine lactating mammary gland are able to synthesize protein, whereas similar preparations from non-lactating glands are not. Washing the ribosome suspensions through a medium containing 0.5m-ammonium chloride enhanced their ability to incorporate phenylalanine into polyphenylalanine. 2. Ribosomes isolated from non-lactating bovine mammary gland, in contrast with those from rat liver and lactating mammary gland, contained significant amounts of extraneous nucleases. These enzymes could be removed by washing with a medium A buffer containing 0.5m-ammonium chloride. 3. Only those ribosomes from functionally active tissues were able to bind polyuridylic acid and phenylalanyl-tRNA.
摘要
- 从牛乳腺中制备的核糖体能够合成蛋白质，而从非泌乳期乳腺中制备的类似核糖体则不能。通过含有0.5M氯化铵的介质洗涤核糖体悬浮液可增强其将苯丙氨酸掺入多聚苯丙氨酸的能力。2. 与从大鼠肝脏和泌乳期乳腺中分离的核糖体相比，从非泌乳期牛乳腺中分离的核糖体含有大量的外源核酸酶。这些酶可以通过用含有0.5M氯化铵的介质A缓冲液洗涤来去除。3. 只有来自功能活跃组织的那些核糖体能够结合聚尿苷酸和苯丙氨酰 - tRNA。
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