Henke Lotte, Muche Matthias, Blaauw André, Van Eede Peter H, Martin Wolfgang, Helmken Claudia, Budowle Bruce, Henke Juergen
Institut für Blutgruppenforschung LGC GmbH, Köln, Germany.
Clin Lab. 2007;53(7-8):477-82.
The Humantype Chimera PCR Amplification Kit contains 12 polymorphic loci (ACTBP2 (= SE33), D18S51, D4S2366, D6S474, D8S1132, D12S391, D2S1360, D3S1744, D5S2500, D7S1517, D10S2325, D21S2055), of which the latter 10 loci have not been used extensively for human identity testing. The sex determinant locus amelogenin is also included in the kit. Amplification was successful on a variety of thermal cyclers and the amplicons could be analyzed on both the ABI PRISM 310 and 3100 Genetic Analyzers. Complete genotyping results from single source samples were possible between 0.25 and 2 ng of DNA template. Heterozygote imbalance (< 60% peak height balance) caused by stochastic effects was observed at a rate of around 5%. No deviations from the Hardy-Weinberg equilibrium were observed. Thus, there were no detectable significant deviations from the expected genetic independence of alleles.
人类类型嵌合体PCR扩增试剂盒包含12个多态性位点（ACTBP2（=SE33）、D18S51、D4S2366、D6S474、D8S1132、D12S391、D2S1360、D3S1744、D5S2500、D7S1517、D10S2325、D21S2055），其中后10个位点尚未广泛用于人类身份鉴定测试。试剂盒中还包括性别决定位点牙釉蛋白。在多种热循环仪上扩增均成功，扩增产物可在ABI PRISM 310和3100基因分析仪上进行分析。DNA模板量在0.25至2 ng之间时，单一样本的完整基因分型结果是可行的。由随机效应导致的杂合子失衡（峰高平衡<60%）发生率约为5%。未观察到偏离哈迪-温伯格平衡的情况。因此，未检测到等位基因预期遗传独立性的显著偏差。
Zotero 插件