[将从抗水稻细菌性条斑病的玉米中克隆的非宿主基因Rxo1导入水稻品种的研究]

[Introduction of a non-host gene Rxo1 cloned from maize resistant to rice bacterial leaf streak into rice varieties].

作者信息

Xie Xue-Wen, Yu Jing, Xu Jian-Long, Zhou Yong-Li, Li Zhi-Kang

机构信息

Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2007 Jul;23(4):607-11. doi: 10.1016/s1872-2075(07)60039-9.

DOI:10.1016/s1872-2075(07)60039-9

PMID:17822031

Abstract

Rice bacterial leaf streak,caused by Xanthomonas oryzae pv. oryzicola is a destructive bacterial disease in China. Single-gene resistance to X. oryzae pv. oryzicola has not been found in rice germplasm. A cloned non-host gene from maize with resistance to bacterial leaf streak, Rxo1, was transferred into four Chinese rice varieties through an Agrobacterium-mediated system, including Zhonghua11, 9804, C418 and Minghui86. PCR and Southern analysis of the transgenic plants revealed the integration of the Rxo1 gene into the rice genomes. The integrated Rxo1 was stably inherited, and segregated in a 3:1 (Resistance:Susceptible) ratio in the selfed T1 generations derived from some T0 plants, indicating that Rxo1 inherited as a dominate gene in rice. Transgenic T0 plants and PCR-positive T1 plants were resistant to X. oryzae pv. oryzicola on the basis of artificial inoculation.

摘要

水稻细菌性条斑病由水稻条斑病菌引起，是中国一种具有毁灭性的细菌性病害。在水稻种质资源中尚未发现对水稻条斑病菌的单基因抗性。一个从玉米中克隆的对细菌性条斑病具有抗性的非寄主基因Rxo1，通过农杆菌介导系统转入了四个中国水稻品种，包括中华11、9804、C418和明恢86。对转基因植株的PCR和Southern分析表明Rxo1基因已整合到水稻基因组中。整合的Rxo1能够稳定遗传，并且在一些T0植株衍生的自交T1代中以3:1（抗性:感病）的比例分离，这表明Rxo1在水稻中作为显性基因遗传。基于人工接种，转基因T0植株和PCR阳性T1植株对水稻条斑病菌具有抗性。