Klein R, Tatischeff I, Tham G, Grolière C A
Institut Curie, CNRS, URA 198, Paris, France.
Biochimie. 1991 Oct;73(10):1281-5. doi: 10.1016/0300-9084(91)90089-j.
The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence properties and by its oxidation product with alkaline permanganate. The final identification was obtained by comparison with an authentic material using an exchange ligand chromatography method with D-phenylalanine as chiral modifier and Cu (II) as metal ion. D-monapterin is also present as the major pterin in Tetrahymena pyriformis strains GL and ST, and in Tetrahymena thermophila.
梨形四膜虫W株中的主要蝶呤，先前认为是L-苏型生物蝶呤并命名为纤毛蝶呤[1]，现在被鉴定为D-苏型新蝶呤（D-单蝶呤）。这是天然D-单蝶呤的首个实例。该化合物通过其色谱行为、荧光特性以及其碱性高锰酸钾氧化产物进行表征。最终鉴定是通过使用以D-苯丙氨酸作为手性改性剂和Cu（II）作为金属离子的交换配体色谱法与纯品进行比较而获得的。D-单蝶呤在梨形四膜虫GL株和ST株以及嗜热栖热四膜虫中也作为主要蝶呤存在。
