Franzini C, Cattozzo G, Ceriotti F, Ferrero C A, Berti P, Bianchi P, Bonvicini P, Ceriotti G, Deledda R, Fenili D
Ospedale di Rho, Milano, Italy.
Eur J Clin Chem Clin Biochem. 1991 Nov;29(11):759-65.
Some aspects of the measurement of alkaline phosphatase activity concentration in human serum, using N-methyl-D-glucamine as a buffer, were evaluated with a view to the possible routine use of the method. The evaluated characteristics included: the temperature-dependence of the pH of the buffer; the effect of adding the magnesium/zinc ions buffer; the effect of the serum volume fraction; the substrate-starter versus the serum-starter mode; the effect of modifying the formulation of reagents; the within-run and the between laboratory imprecision; the correlation with an alternative routine method. Also, sex- and age-related reference values were produced, based on 2968 values from selected reference sample groups in 7 laboratories. In general, the results demonstrate the robustness of the method, its adaptability to a variety of mechanized analysers, and hence its feasibility as a routine measurement method.
为了评估该方法在常规应用中的可能性，我们对以N-甲基-D-葡糖胺作为缓冲剂测定人血清碱性磷酸酶活性浓度的某些方面进行了评估。评估的特性包括：缓冲液pH值的温度依赖性；添加镁/锌离子缓冲液的影响；血清体积分数的影响；底物启动模式与血清启动模式；试剂配方修改的影响；批内和实验室间的不精密度；与另一种常规方法的相关性。此外，基于7个实验室选定参考样本组的2968个值，得出了与性别和年龄相关的参考值。总体而言，结果证明了该方法的稳健性、对各种自动化分析仪的适应性，因此其作为常规测量方法的可行性。
