Kamath Sudha D, Kartha V B, Mahato K K
Centre for Laser Spectroscopy, KMC Life Sciences Centre, Manipal Academy of Higher Education (Deemed University), Manipal 576 104, Karnataka, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Jun;70(1):187-94. doi: 10.1016/j.saa.2007.06.041. Epub 2007 Jul 28.
An experimental system for measuring simultaneously photoacoustic (PA) and fluorescence signals is described. The simultaneous measurement of laser induced fluorescence and photoacoustic signals provide a suitable method for the study of different quenching phenomena occurring in fluorescent systems. In this paper we report tryptophan solvation dynamics in water using fluorescence and photoacoustic spectra recorded simultaneously by photoacoustic and fluorescence signals as functions of concentration, indicate that quantum yield is maximum at low concentrations. Also, the energy lost in the fluorescence path of tryptophan, due to different quenching phenomena like self quenching, Resonance energy transfer (RET), solvation relaxation, etc. is clearly seen from the photoacoustic signal intensity which increases as the fluorescence intensity decreases.
描述了一种用于同时测量光声（PA）和荧光信号的实验系统。激光诱导荧光和光声信号的同时测量为研究荧光系统中发生的不同猝灭现象提供了一种合适的方法。在本文中，我们利用光声和荧光信号同时记录的荧光和光声光谱，报告了水中色氨酸的溶剂化动力学，该光谱作为浓度的函数，表明量子产率在低浓度时最大。此外，从光声信号强度可以清楚地看到，由于不同的猝灭现象，如自猝灭、共振能量转移（RET）、溶剂化弛豫等，色氨酸在荧光路径中损失的能量随着荧光强度的降低而增加。
