Chegini N, Lei Z M, Rao C V, Hansel W
Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Kentucky 40292.
Biol Reprod. 1991 Sep;45(3):506-13. doi: 10.1095/biolreprod45.3.506.
Bovine luteal functions are regulated by gonadotropins and eicosanoids. The specific binding sites that presumably mediate the actions of these regulatory agents have previously been characterized in bovine luteal tissue. However, the cellular distribution and/or the cycle phase dependency of these binding sites have never been investigated. In the present study, we investigated these parameters by using quantitative light microscope autoradiography. The results showed that both small and large luteal cells contained binding sites for LH/hCG, prostaglandin (PG)E2, PGF2 alpha, PGI2, and leukotriene (LT)C4. In addition, luteal blood vessels contained LH/hCG and LTC4 binding sites and luteal fibroblasts contained PGE2 binding sites. On a per cell basis, there were more binding sites for all ligands in large luteal cells as compared to small or nonluteal cells. After correction for the cellular area differences, small luteal cells contained more LH/hCG, PGE2, PGI2, and LTC4 binding sites, while large luteal cells contained more PGF2 alpha binding sites. The small and large luteal cell binding of hCG, PGE2, PGI2, and LTC4 increased from early to mid luteal phase, followed by a decline in the late luteal phase. PGF2 alpha binding, on the other hand, increased from early to late luteal phase. In contrast to luteal cells, binding of hCG and LTC4 to luteal blood vessels and binding of PGE2 to luteal fibroblasts did not change during the cycle. These results suggest that LH/hCG and eicosanoid regulation of luteal function is more complex than previously envisioned and it involves both small and large luteal cells and, in some cases, also nonluteal cells.
牛黄体功能受促性腺激素和类花生酸调节。推测介导这些调节因子作用的特异性结合位点先前已在牛黄体组织中得到表征。然而，这些结合位点的细胞分布和/或周期阶段依赖性从未被研究过。在本研究中，我们通过定量光学显微镜放射自显影术研究了这些参数。结果表明，小黄体细胞和大黄体细胞均含有促黄体生成素/人绒毛膜促性腺激素（LH/hCG）、前列腺素（PG）E2、PGF2α、前列环素（PGI2）和白三烯（LT）C4的结合位点。此外，黄体血管含有LH/hCG和LTC4结合位点，黄体成纤维细胞含有PGE2结合位点。以每个细胞计算，与小黄体细胞或非黄体细胞相比，大黄体细胞中所有配体的结合位点更多。在对细胞面积差异进行校正后，小黄体细胞含有更多的LH/hCG、PGE2、PGI2和LTC4结合位点，而大黄体细胞含有更多的PGF2α结合位点。hCG、PGE2、PGI2和LTC4在小黄体细胞和大黄体细胞中的结合从黄体早期到中期增加，随后在黄体晚期下降。另一方面，PGF2α的结合从黄体早期到晚期增加。与黄体细胞不同，hCG和LTC4与黄体血管的结合以及PGE2与黄体成纤维细胞的结合在周期中没有变化。这些结果表明，LH/hCG和类花生酸对黄体功能调节比先前设想的更为复杂，它涉及小黄体细胞和大黄体细胞，在某些情况下还涉及非黄体细胞。
