Nicholson Eric M, Kunkle Robert A, Hamir Amir N, Lebepe-Mazur Semakaleng, Orcutt Dennis
Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
J Vet Diagn Invest. 2007 Sep;19(5):548-52. doi: 10.1177/104063870701900515.
Clinical signs of prion disease are not specific and include a variety of differential diagnoses. Serological tests and nucleic acid-based detection methods are not applicable to prion-disease-agent detection because of the unusual nature of the infectious agent. Prion-disease diagnosis is primarily conducted by means of immunodetection of the infectious agent, typically by at least 2 distinct procedures with immunohistochemistry and Western blot being the most informative. These approaches differ in the need for formalin-fixed and frozen or fresh tissue respectively. This work describes a method for the detection of the disease-associated isoform of the prion protein by Western blot using formalin-fixed tissues. The approach requires only minimal modification of existing Western-blot procedures and could readily be incorporated into existing detection schemes for confirmatory purposes when fresh or frozen tissues are unavailable.
朊病毒疾病的临床症状并不具有特异性，包括多种鉴别诊断。由于感染因子的特殊性质，血清学检测和基于核酸的检测方法不适用于朊病毒疾病病原体的检测。朊病毒疾病的诊断主要通过对感染因子进行免疫检测来进行，通常采用至少两种不同的方法，其中免疫组织化学和蛋白质印迹法提供的信息最多。这些方法分别对福尔马林固定组织以及冷冻或新鲜组织有不同需求。本研究描述了一种使用福尔马林固定组织通过蛋白质印迹法检测朊病毒蛋白疾病相关异构体的方法。该方法只需对现有的蛋白质印迹程序进行最小程度的修改，并且在无法获得新鲜或冷冻组织时，可以很容易地纳入现有的检测方案以进行确证。
