Okorokov Andrei L, Waugh Alastair, Hodgkinson Julie, Murthy Andal, Hong Hye Kyung, Leo Elisabetta, Sherman Michael B, Stoeber Kai, Orlova Elena V, Williams Gareth H
Department of Pathology, University College London, London WC1E 6JJ, UK.
EMBO Rep. 2007 Oct;8(10):925-30. doi: 10.1038/sj.embor.7401064. Epub 2007 Sep 7.
The DNA replication factor minichromosome maintenance 10 (MCM10) is a conserved, abundant nuclear protein crucial for origin firing. During the transition from pre-replicative complexes to pre-initiation complexes, MCM10 recruitment to replication origins is required to provide a physical link between the MCM2-7 complex DNA helicase and DNA polymerases. Here, we report the molecular structure of human MCM10 as determined by electron microscopy and single-particle analysis. The MCM10 molecule is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers. This structure, together with biochemical data, suggests that this important protein uses its architecture to provide a docking module for assembly of the molecular machinery required for eukaryotic DNA replication.
DNA复制因子微小染色体维持蛋白10（MCM10）是一种保守的、丰富的核蛋白，对起始点激发至关重要。在从复制前复合物向起始前复合物转变的过程中，MCM10募集到复制起始点是在MCM2-7复合物DNA解旋酶与DNA聚合酶之间建立物理连接所必需的。在此，我们报告了通过电子显微镜和单颗粒分析确定的人MCM10的分子结构。MCM10分子是一个环形六聚体，具有大的中央通道和较小的侧向通道以及一个内腔系统。这种结构与生化数据一起表明，这种重要的蛋白质利用其结构为真核DNA复制所需分子机器的组装提供一个对接模块。
