Makni F, Hachicha L, Mseddi F, Hammami H, Cheikhrouhou F, Sellami H, Sellami A, Mzali R, Boujelbène S, Rebaï R, Beyrouti I, Ayadi A
Laboratoire de parasitologie-mycologie, CHU Habib-Bourguiba, Sfax, Tunisie.
Bull Soc Pathol Exot. 2007 Aug;100(3):171-3.
The aim of this study is to evaluate the contribution of the immunoWesternblot for the diagnosis and the post surgical follow-up of the hydatidosis. 71 sera from patients with hydatidosis confirmed by surgery were studied. All had a negative hydatic serology by screening tests (enzyme-linked immunosorbent assay, hemagglutination, electrosyneresis). 12 patients with sera in pre and post operative were monitored for 2 years. The Echinococcus Western blot IgG permitted to rectify the diagnosis of hydatidosis in 67.6 %. The rate of positivity was 100 % for the multivesicular liver cysts, 60 % for the young cysts and 50 % for the calcified cysts. Western blot permitted to rectify the diagnosis of lung cysts in 62.5 % of cases and in 50 % of cranial-spinal localizations. Analysis of Western Blot evolution in the 12 patients followed in pre and post-surgical revealed the disappearance of the bands 16, 18 and 26-28kDa in 8 month in the 8 patients with complete exeresis. This study proved the value added of Western blot compared to the other traditional techniques for the immunodiagnostic and the post-surgical monitoring of hydatidosis.
本研究的目的是评估免疫印迹法在包虫病诊断及术后随访中的作用。研究了71例经手术确诊为包虫病患者的血清。所有患者经筛查试验（酶联免疫吸附测定、血凝试验、电凝聚试验）包虫血清学均为阴性。对12例术前和术后血清患者进行了2年的监测。细粒棘球绦虫免疫印迹IgG可纠正67.6%的包虫病诊断。多囊性肝囊肿的阳性率为100%，年轻囊肿为60%，钙化囊肿为50%。免疫印迹法可纠正62.5%的肺囊肿病例及50%的颅-脊髓部位病例的诊断。对12例术前和术后随访患者的免疫印迹演变分析显示，8例完全切除的患者在8个月内16、18和26 - 28kDa条带消失。本研究证明了与其他传统技术相比，免疫印迹法在包虫病免疫诊断和术后监测中的附加价值。
