Stein Aaron
GIBCO Cell Culture Systems, Invitrogen.
Biotechniques. 2007 Aug;43(2):228-9. doi: 10.2144/000112561.
Culturing mammalian cells has not significantly changed in almost 50 years. Typically, a synthetic basal medium is chosen to meet the environmental and nutritional requirements of a given cell line. Components, such as amino acids, vitamins, inorganic salts, and a carbon source such as glucose are commonly found in the classical basal media formulation. These basal formulations normally will not support cell growth alone, but must be further supplemented with animal serum, usually fetal bovine serum (FBS) at a concentration of 5-20%. Recent advances in serum-free and chemically defined media formulations have provided cell culturists with options. When considering FDA regulations and potential risks to human health when manufacturing biologics or considering cell therapies, eliminating serum is of paramount concern. For a large majority of researchers however, using classical media with serum builds on previous generations of research and makes cell culture easier to perform.
近50年来，哺乳动物细胞培养技术并没有显著变化。通常，会选择一种合成基础培养基来满足特定细胞系的环境和营养需求。经典基础培养基配方中常见的成分包括氨基酸、维生素、无机盐以及如葡萄糖这样的碳源。这些基础配方通常不能单独支持细胞生长，而是必须进一步添加动物血清，通常是浓度为5%-20%的胎牛血清（FBS）。无血清和化学成分确定的培养基配方的最新进展为细胞培养人员提供了更多选择。在考虑制造生物制品或细胞疗法时的FDA法规以及对人类健康的潜在风险时，去除血清是至关重要的。然而，对于绝大多数研究人员来说，使用含血清的经典培养基是基于前几代的研究，并且使细胞培养操作更简便。
