Oztürk Nevra, Günay M Emin, Akgöl Sinan, Denizli Adil
Chemistry Department, Adnan Menderes University, Aydin, Turkey.
Biotechnol Prog. 2007 Sep-Oct;23(5):1149-56. doi: 10.1021/bp070158o. Epub 2007 Sep 7.
The magnetic poly(2-hydroxyethyl methacrylate ethylene glycol dimethacrylate) [m-poly(HEMA-EGDMA)] beads (150-250-microm diameter in spherical form) were prepared by a radical suspension polymerization technique. The pseudo-specific ligand, reactive imidazole containing 3-(2-imidazoline-1-yl)propyl (triethoxysilane) (IMEO) was selected as a silanization agent. IMEO was covalently immobilized onto the magnetic beads. IMEO-immobilized m-poly(HEMA-EGDMA) beads were used for the affinity adsorption of immunoglobulin-G (IgG) from aqueous solutions and human plasma. To evaluate the degree of IMEO attachment, the m-poly(HEMA-EGDMA) beads were subjected to Si analysis by using flame atomizer atomic absorption spectrometer, and it was estimated as 36.6 mg IMEO/g of polymer. The nonspecific IgG adsorption onto the plain m-poly(HEMA-EGDMA) beads was very low (about 0.4 mg/g). Higher adsorption values (up to 55 mg/g) were obtained when the m-poly(HEMA-EGDMA)/IMEO beads were used from both aqueous solutions and human plasma. The maximum IgG adsorption on the m-poly(HEMA-EGDMA)-IMEO beads was observed at pH 7.0. The IgG molecules could be repeatedly adsorbed and desorbed with m-poly(HEMA-EGDMA)-IMEO beads without noticeable loss in the IgG adsorption capacity. The adsorption capacity from human plasma in magnetically stabilized fluidized bed decreased drastically from 78.9 to 19.6 mg/g with the increase of the flow rate from 0.2 to 3.5 mL/min.
通过自由基悬浮聚合技术制备了磁性聚（甲基丙烯酸2-羟乙酯-乙二醇二甲基丙烯酸酯）[m-聚（HEMA-EGDMA）]珠粒（球形，直径150 - 250微米）。选择含有3-(2-咪唑啉-1-基)丙基（三乙氧基硅烷）（IMEO）的假特异性配体反应性咪唑作为硅烷化剂。IMEO共价固定在磁珠上。固定有IMEO的m-聚（HEMA-EGDMA）珠粒用于从水溶液和人血浆中亲和吸附免疫球蛋白G（IgG）。为了评估IMEO的附着程度，使用火焰原子化器原子吸收光谱仪对m-聚（HEMA-EGDMA）珠粒进行硅分析，估计为36.6 mg IMEO/g聚合物。普通m-聚（HEMA-EGDMA）珠粒对IgG的非特异性吸附非常低（约0.4 mg/g）。当使用m-聚（HEMA-EGDMA）/IMEO珠粒从水溶液和人血浆中吸附时，获得了更高的吸附值（高达55 mg/g）。在pH 7.0时观察到m-聚（HEMA-EGDMA）-IMEO珠粒对IgG的最大吸附。IgG分子可以用m-聚（HEMA-EGDMA）-IMEO珠粒反复吸附和解吸，而IgG吸附容量没有明显损失。在磁稳定流化床中，随着流速从0.2 mL/min增加到3.5 mL/min，人血浆中的吸附容量从78.9 mg/g急剧下降到19.6 mg/g。
