Monton Maria Rowena N, Tomita Masaru, Soga Tomoyoshi, Ishihama Yasushi
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
Anal Chem. 2007 Oct 15;79(20):7838-44. doi: 10.1021/ac071038y. Epub 2007 Sep 8.
An easy-to-implement capillary coating strategy based on polymer entrapment in the network of polymerized silicate is described. In this manner, cationic polymers are tightly fixed onto the inner wall of the capillary for electroosmotic flow control without necessitating complex surface modification chemistries. The resulting coated capillary exhibited good stability over a wide range of pH, good reproducibility, strong endurance in more than 300 electrophoretic runs, and tolerance of commonly employed organic solvent additives in CE. Applications in CE-MS analysis of biologically important anions as well as sample enrichment are shown. Additionally, it was used as a durable base for attachment of multiple layers of charged polymers on the wall, via electrostatic interaction with the preceding layer. Thus, two novel types of highly stable coated capillaries, one with anodic EOF and the other cathodic, were developed.
本文描述了一种基于聚合物截留在聚合硅酸盐网络中的易于实施的毛细管涂层策略。通过这种方式，阳离子聚合物被紧密固定在毛细管内壁上，用于电渗流控制，而无需复杂的表面改性化学方法。所得的涂层毛细管在很宽的pH范围内表现出良好的稳定性、良好的重现性、在300多次电泳运行中具有很强的耐久性，并且对毛细管电泳中常用的有机溶剂添加剂具有耐受性。展示了其在毛细管电泳-质谱联用分析生物重要阴离子以及样品富集方面的应用。此外，它还作为通过与前一层的静电相互作用在壁上附着多层带电聚合物的耐用基底。因此，开发了两种新型的高度稳定的涂层毛细管，一种具有阳极电渗流，另一种具有阴极电渗流。
