Tomaino Barbara, Cappello Paola, Capello Michela, Fredolini Claudia, Ponzetto Antonio, Novarino Anna, Ciuffreda Libero, Bertetto Oscar, De Angelis Claudio, Gaia Enzo, Salacone Paola, Milella Michele, Nisticò Paola, Alessio Massimo, Chiarle Roberto, Giuffrida Maria G, Giovarelli Mirella, Novelli Francesco
Center for Experimental Research and Medical Studies and University of Turin, San Giovanni Battista Hospital, Turin, Italy.
J Proteome Res. 2007 Oct;6(10):4025-31. doi: 10.1021/pr070281a. Epub 2007 Sep 8.
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy characterized by rapid progression, invasiveness, and resistance to treatment. It is the fourth leading cause of cancer death with a 2% 5-year survival rate. Biomarkers for its early detection are lacking. This study was designed to use a proteomics-based approach as a means of identifying antigens that elicit a humoral response in PDAC patients. Antibodies against PDAC-associated antigens are useful for early cancer diagnosis and therapy. Proteins from PDAC cell lines were separated by 2-DE, and the serum IgG reactivity of 70 PDAC patients, 40 healthy subjects (HS), 30 non-PDAC tumor patients, and 15 chronic pancreatitis (CP) patients was tested by Western blot analysis. Spots specifically recognized by PDAC sera and revealed by mass spectrometry corresponded to metabolic enzymes or cytoskeletal proteins. Most were up-regulated in PDAC tissues. Thus, it seems that metabolic enzymes and cytoskeletal proteins are specific targets of the humoral response during PDAC. The results of further studies of these serological-defined antigens could be of diagnostic and therapeutic significance in PDAC.
胰腺导管腺癌（PDAC）是一种极具侵袭性的恶性肿瘤，其特点是进展迅速、具有侵袭性且对治疗有抗性。它是癌症死亡的第四大主要原因，5年生存率仅为2%。目前缺乏用于早期检测的生物标志物。本研究旨在采用基于蛋白质组学的方法来识别在PDAC患者中引发体液免疫反应的抗原。针对PDAC相关抗原的抗体对癌症早期诊断和治疗有用。通过二维电泳分离PDAC细胞系的蛋白质，并用蛋白质印迹分析检测70例PDAC患者、40例健康受试者（HS）、30例非PDAC肿瘤患者和15例慢性胰腺炎（CP）患者血清IgG的反应性。经质谱鉴定，PDAC血清特异性识别并显示的斑点对应于代谢酶或细胞骨架蛋白。大多数在PDAC组织中上调。因此，代谢酶和细胞骨架蛋白似乎是PDAC期间体液免疫反应的特异性靶点。对这些血清学定义抗原的进一步研究结果可能对PDAC具有诊断和治疗意义。
