[与NF-κB p50亚基Rel同源结构域相互作用的多肽筛选]
[Screening of polypeptides interacting with Rel homology domain of NF-kappaB p50 subunit].
作者信息
Shen Li-qun, Xu Xiang, Liang Hua-ping, Li Sheng-mao
机构信息
Research Institute of Surgery, Daping Hospital, Chongqing 400042, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Sep;23(9):804-6.
AIM
To obtain the polypeptides interacting with NF-kappaB p50 Rel homology domain (RHD) by yeast two-hybrid technique.
METHODS
Using NF-kappaB p50 RHD as bait, the polypeptides interacting with NF-kappaB p50 RHD were screened from a 16-peptide cDNA library by yeast two-hybrid technique. The false positive clones were screened out but the positive clone were identified by beta-GAL assay, yeast mating, and one to one yeast two-hybrid method.
RESULTS
8 positive clones were obtained. The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank.
CONCLUSION
8 novel polypeptides interacting with NF-kappaB p50 RHD were obtained, but their functions need to be validated in further experiments.
目的
通过酵母双杂交技术获得与核因子-κB p50 蛋白Rel同源结构域（RHD）相互作用的多肽。
方法
以核因子-κB p50 RHD为诱饵，利用酵母双杂交技术从一个16肽cDNA文库中筛选与核因子-κB p50 RHD相互作用的多肽。通过β-半乳糖苷酶检测、酵母交配和一对一酵母双杂交方法筛选出假阳性克隆并鉴定阳性克隆。
结果
获得8个阳性克隆。这8种多肽的基因序列互不相同，在GenBank中未发现其同源蛋白。
结论
获得了8种与核因子-κB p50 RHD相互作用的新型多肽，但其功能有待进一步实验验证。
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