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[中国健康个体外周血中CD4+CD25nt/hiCD127lo调节性T淋巴细胞的频率]

[Frequency of the CD4+CD25nt/hiCD127lo regulatory T lymphocytes from the peripheral blood in Chinese healthy individuals].

作者信息

Wang Ying, Zhou Lei-ming, Gong Ju-li, Zhou Ming-kui, Shen Fang-wei, Zheng Xiao-hong, Zhang Wei, Zhuang Ming-hua, Lu Wei, Zhong Ping, Pan Qi-chao, Kang Lai-yi

机构信息

Shanghai Municipal Center for Disease Control and Prevention, Shanghai Municipal Center for AIDS Research Director, Shanghai 200336, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Sep;23(9):816-8.

PMID:17825226
Abstract

AIM

To determine the frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals and provide some useful evidence for clinical research of correlative diseases.

METHODS

From the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes of peripheral blood in 312 Chinese healthy male and female individuals aged from 8 to 60(five age groups were collected) The expression of transcription factor Foxp3 was detected by triplex immuno fluorescence and the frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes was determined by flow cytometry.

RESULTS

The frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes in Chinese healthy individuals was (6.55+/-0.11)%, and the frequency differed among age groups(P=0.015) and sex groups(P<0.05). CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes specifically express transcription factor Foxp3.

CONCLUSION

The frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals has been preliminarily determined which lays the foundation for further clinical research of regulatory T lymphocytes. As a specific cell surface marker, CD25(nt/hi)CD127(lo) can helpful obtain pure CD4(+)CD25(+) regulatory T lymphocytes and suppress the interference of other cells during cell separation.

摘要

目的

测定中国健康人群外周血中CD4(+)CD25(nt/hi)CD127(lo)调节性T淋巴细胞的频率，为相关疾病的临床研究提供有用依据。

方法

收集312名年龄在8至60岁之间的中国健康男女（分为五个年龄组）外周血中的CD4(+)CD25(nt/hi)CD127(lo)调节性T淋巴细胞，采用三重免疫荧光法检测转录因子Foxp3的表达，并用流式细胞术测定CD4(+)CD25(nt/hi)CD127(lo)调节性T淋巴细胞的频率。

结果

中国健康人群中CD4(+)CD25(nt/hi)CD127(lo)调节性T淋巴细胞的频率为(6.55±0.11)%，不同年龄组间频率有差异（P=0.015），不同性别组间频率也有差异（P<0.05）。CD4(+)CD25(nt/hi)CD127(lo)调节性T淋巴细胞特异性表达转录因子Foxp3。

结论

初步测定了中国健康人群外周血中CD4(+)CD25(nt/hi)CD127(lo)调节性T淋巴细胞的频率，为调节性T淋巴细胞的进一步临床研究奠定了基础。作为一种特异性细胞表面标志物，CD25(nt/hi)CD127(lo)有助于获得纯的CD4(+)CD25(+)调节性T淋巴细胞，并在细胞分离过程中抑制其他细胞的干扰。

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