Liu Yang, Zhang Hong, Zhang Luwei, Zhou Qingming, Wang Xiaohu, Long Jing, Dong Tao, Zhao Weiping
Department of Medical Physics, Institute of Modern physics, Chinese Academy of Sciences, Lanzhou, China.
Eur J Pharmacol. 2007 Dec 1;575(1-3):142-8. doi: 10.1016/j.ejphar.2007.07.026. Epub 2007 Jul 21.
The aim of this study was to evaluate the protective effects of different doses and administration modes of N-acetylcysteine (NAC) against X-ray -induced liver damage in mice. Kun-Ming mice were divided into four groups, each composed of six animals: two control groups and two NAC-treated groups. An acute study was carried out to determine alterations in lipid peroxidation (determined by measuring malondiadehyde (MDA) level), glutathione (GSH) content and superoxide dismutase (SOD) activity (assayed by colorimetric method), and DNA damage (characterized by DNA-single strand break using with comet assay) as well as cell apoptosis (measured by flow cytometry) at 12 h after irradiation. The results showed that there were dose-related decreases in MDA level, DNA damage and cell apoptosis, and dose-dependent increases in GSH content and SOD activity in all NAC-treated groups compared to control groups, indicating that pre-treatment or post-treatment with NAC significantly attenuates the acute liver damage caused by X-ray. In addition, significant positive correlations were observed between MDA level and DNA damage or cell apoptosis, implying that lipid peroxidation plays a major role in X-ray-induced liver injury. The data suggest that NAC exerts its radioprotective effect by counteracting accumulated reactive oxygen species in the liver through its properties as a direct antioxidant and a GSH precursor, when administered before or after X-ray irradiation.
本研究旨在评估不同剂量和给药方式的N-乙酰半胱氨酸（NAC）对X射线诱导的小鼠肝脏损伤的保护作用。昆明小鼠分为四组，每组六只动物：两个对照组和两个NAC处理组。进行了一项急性研究，以确定照射后12小时脂质过氧化（通过测量丙二醛（MDA）水平来确定）、谷胱甘肽（GSH）含量和超氧化物歧化酶（SOD）活性（采用比色法测定）的变化，以及DNA损伤（通过彗星试验以DNA单链断裂为特征）和细胞凋亡（通过流式细胞术测量）。结果表明，与对照组相比，所有NAC处理组的MDA水平、DNA损伤和细胞凋亡均呈剂量依赖性降低，而GSH含量和SOD活性呈剂量依赖性增加，这表明NAC预处理或后处理可显著减轻X射线引起的急性肝损伤。此外，MDA水平与DNA损伤或细胞凋亡之间存在显著正相关，这意味着脂质过氧化在X射线诱导的肝损伤中起主要作用。数据表明，在X射线照射之前或之后给药时，NAC通过作为直接抗氧化剂和GSH前体的特性来对抗肝脏中积累的活性氧，从而发挥其辐射防护作用。
