钌（II）混合配体配合物[Ru(phen)2(dtmi)]2+和[Ru(phen)2(dtni)]2+的DNA结合相互作用研究
Interaction studies of DNA binding of ruthenium(II) mixed-ligand complexes: [Ru(phen)2(dtmi)]2+ and [Ru(phen)2(dtni)]2+.
作者信息
Liu Yun-Jun, Wei Xin Yu, Wu Fu-Hai, Mei Wen-Jie, He Li-Xin
机构信息
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Jun;70(1):171-6. doi: 10.1016/j.saa.2007.07.029. Epub 2007 Jul 27.
Two new ligands, 3-(pyrazin-2-yl)-as-triazino[5,6-f]-5-methoxylisatin (dtmi), 3-(pyrazin-2-yl)-as-triazino[5,6-f]-5-nitroisatin (dtni) and their ruthenium(II) complexes Ru(phen)2(dtmi)2 (1) and Ru(phen)2(dtni)2 (2) have been prepared and characterized by elemental analysis, FAB-MS, ES-MS and 1H NMR. The DNA-binding behaviors of complexes have been studied by spectroscopic titration, viscosity measurements, thermal denaturation and circular dichromism (CD). The results indicate that the complexes 1 and 2 interact with calf thymus DNA (CT-DNA) by intercalative mode. The DNA-binding affinity of the complexes 2 is larger than that complex 1 does.
制备了两种新的配体，3-(吡嗪-2-基)-三嗪并[5,6-f]-5-甲氧基异吲哚酮（dtmi）、3-(吡嗪-2-基)-三嗪并[5,6-f]-5-硝基异吲哚酮（dtni）及其钌（II）配合物Ru(phen)2(dtmi)2（1）和Ru(phen)2(dtni)2（2），并通过元素分析、快原子轰击质谱（FAB-MS）、电喷雾质谱（ES-MS）和1H核磁共振（1H NMR）对其进行了表征。通过光谱滴定、粘度测量、热变性和圆二色性（CD）研究了配合物的DNA结合行为。结果表明，配合物1和2通过插入模式与小牛胸腺DNA（CT-DNA）相互作用。配合物2与DNA的结合亲和力大于配合物1。
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