Gazola Vilma A F G, Garcia Rosângela F, Hartmann Eduardo M, Barrena Helenton C, Albuquerque Gabriela G, Souza Helenir M, Bazotte Roberto B
Department of Morphophysiological Sciences, State University of Maringá, Maringá, PR, Brazil.
J Diabetes Complications. 2007 Sep-Oct;21(5):320-5. doi: 10.1016/j.jdiacomp.2006.07.002.
The acute effects of the oral administration of L-alanine (L-ala), L-glutamine (L-gln), L-ala+L-gln, and L-alanyl-L-glutamine (AGP) on glycemia recovery during short-term insulin-induced hypoglycemia (IIH) were compared.
For this purpose, the blood glucose levels of 24-h-fasted rats that received intraperitoneal injections of regular insulin (IIH group) or saline [control (COG) group] and, 15 min later, oral administration of L-ala (100 mg/kg), L-gln (100 mg/kg), L-ala (50 mg/kg)+L-gln (50 mg/kg), or AGP (100 mg/kg) were compared. Liver perfusion experiments and blood collection to measure blood glucose levels were performed 30 min after insulin (1.0 U/kg) or saline injection. Livers from the IIH and COG groups were perfused with saturating concentrations of L-ala, L-gln, L-ala+L-gln, or AGP, and the maximal hepatic production of glucose, urea, ammonia, L-lactate, and pyruvate was evaluated.
In contrast with L-gln, L-ala+L-gln, or AGP, the oral administration of L-ala promoted glycemia recovery. In agreement with these results, livers from IIH rats showed maximal hepatic production of glucose and urea from L-ala with 50% of the amount used to obtain the maximal hepatic production of glucose and urea in livers from COG rats. In contrast with L-gln, L-ala+L-gln, or AGP, the maximal hepatic production of urea from L-ala occurred in the absence of ammonia accumulation.
The results indicate that the best glycemia recovery promoted by the oral administration of L-ala was a consequence of the higher efficiency of the livers from IIH rats in producing glucose from L-ala.
比较口服L-丙氨酸（L-ala）、L-谷氨酰胺（L-gln）、L-ala+L-gln和L-丙氨酰-L-谷氨酰胺（AGP）对短期胰岛素诱导低血糖（IIH）期间血糖恢复的急性影响。
为此，比较了24小时禁食大鼠的血糖水平，这些大鼠接受腹腔注射正规胰岛素（IIH组）或生理盐水[对照组（COG组）]，15分钟后口服L-ala（100毫克/千克）、L-gln（100毫克/千克）、L-ala（50毫克/千克）+L-gln（50毫克/千克）或AGP（100毫克/千克）。在胰岛素（1.0单位/千克）或生理盐水注射30分钟后进行肝脏灌注实验并采集血液以测量血糖水平。用饱和浓度的L-ala、L-gln、L-ala+L-gln或AGP灌注IIH组和COG组的肝脏，并评估肝脏中葡萄糖、尿素、氨、L-乳酸和丙酮酸的最大生成量。
与L-gln、L-ala+L-gln或AGP相比，口服L-ala可促进血糖恢复。与这些结果一致，IIH大鼠的肝脏显示从L-ala生成葡萄糖和尿素的最大肝脏生成量，所用的量为COG组大鼠肝脏中获得葡萄糖和尿素最大肝脏生成量所用量的50%。与L-gln、L-ala+L-gln或AGP相比，L-ala生成尿素的最大肝脏生成量发生在无氨积累的情况下。
结果表明，口服L-ala促进的最佳血糖恢复是IIH大鼠肝脏从L-ala生成葡萄糖效率更高的结果。
