Zhao Le, Chen Wei, Taylor Kathryn M, Cai Bin, Li Xu
Center for Laboratory Medicine, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, China.
Biochem Biophys Res Commun. 2007 Nov 9;363(1):82-8. doi: 10.1016/j.bbrc.2007.08.127. Epub 2007 Aug 30.
It was reported that expression of the estrogen-regulated zinc transporter LIV-1 was particularly high in human cervical cancer cell line HeLa. This result prompted us to study the role that LIV-1 played in human cervical cancer. The results of real-time PCR showed that LIV-1 mRNA was significantly higher in cervical cancer in situ than in normal tissues. RNAi mediated suppression of LIV-1 in HeLa cells significantly inhibited cell proliferation, colony formation, migration, and invasive ability, but had no effect on cell apoptosis. Furthermore, LIV-1 suppression is accompanied by down-regulation of p44/42 MAPK, phospho-p44/42 MAPK, Snail and Slug expression levels. Hence, our data provide the first evidence that LIV-1 mRNA is overexpressed in cervical cancer in situ and is involved in invasion of cervical cancer cells through targeting MAPK-mediated Snail and Slug expression.
据报道，雌激素调节的锌转运蛋白LIV-1在人宫颈癌细胞系HeLa中的表达特别高。这一结果促使我们研究LIV-1在人类宫颈癌中所起的作用。实时PCR结果显示，原位宫颈癌中LIV-1 mRNA显著高于正常组织。RNAi介导的HeLa细胞中LIV-1的抑制显著抑制了细胞增殖、集落形成、迁移和侵袭能力，但对细胞凋亡没有影响。此外，LIV-1抑制伴随着p44/42 MAPK、磷酸化p44/42 MAPK、Snail和Slug表达水平的下调。因此，我们的数据首次证明LIV-1 mRNA在原位宫颈癌中过表达，并通过靶向MAPK介导的Snail和Slug表达参与宫颈癌细胞的侵袭。
