Kechagia Nektaria, Nicolaou Chryssoula, Ioannidou Vasiliki, Kourti Erieta, Ioannidis Anastassios, Legakis Nicolaos John, Chatzipanagiotou Stylianos
Athens Medical School, Aeginition Hospital, Department of Biopathology and Clinical Microbiology, Vass. Sophias av. 72-74, 115 28, Athens, Greece.
Int J Food Microbiol. 2007 Sep 30;118(3):326-31. doi: 10.1016/j.ijfoodmicro.2007.07.044. Epub 2007 Aug 6.
The distribution of Yersinia strains in animal reservoirs was examined in 835 food animals (pigs, chickens, sheep, cows) from different Greek departments (Attica, Fthiotida, Viotia and Evia) over a one year period. The isolated strains were characterized with respect to the presence of chromosomal (yst) and plasmid-encoded virulence determinants (virF, yadA) and their antimicrobial susceptibility was tested. In total, Yersiniaspp. were obtained from 9.94% of the 835 food animals at slaughter that were sampled in this study. There was no statistically significant seasonal distribution, nor was any significant departmental distribution observed. From the 83 isolated Yersinia strains, 76 (91,57%) belonged to Y. enterocolitica (58 were of serotype O:3/biotype 4 and 18 strains were non O:3, non O:9), 3 belonged to Y. pseudotuberculosis, 2 to Y. kristensenii and 2 to Y. intermedia. Y. enterocolitica O:3/4 was mainly isolated from the pigs, while Y. enterocolitica non O:3, non O:9 was from the chickens. The strains were grouped into 5 genotypes, with respect to the presence or absence of the virulence genes. A significant predominance of genotype V, the one carrying all the three virulence genes, was observed in the strains isolated from the pigs. Complete susceptibility to most of the 3rd and to the 4th generation cephalosporins and to ciprofloxacin, was observed among the isolates. Remarkable was the association between the presence of each virulence gene separately and resistance to some antimicrobials, a matter of further investigation.
在一年时间里，对来自希腊不同地区（阿提卡、弗西奥蒂达、维奥蒂亚和埃维亚）的835头食用动物（猪、鸡、羊、牛）进行了耶尔森菌菌株在动物宿主中的分布情况检查。对分离出的菌株进行了染色体（yst）和质粒编码的毒力决定因素（virF、yadA）的特征分析，并测试了它们的抗菌药敏性。在本研究中，从835头屠宰时采样的食用动物中，共有9.94%检测出耶尔森菌属。未观察到统计学上显著的季节分布，也未发现明显的地区分布差异。在83株分离出的耶尔森菌菌株中，76株（91.57%）属于小肠结肠炎耶尔森菌（58株为O:3血清型/生物型4，18株为非O:3、非O:9），3株属于假结核耶尔森菌，2株属于克氏耶尔森菌，2株属于中间耶尔森菌。小肠结肠炎耶尔森菌O:3/4主要从猪中分离得到，而非O:3、非O:9的小肠结肠炎耶尔森菌则从鸡中分离得到。根据毒力基因的有无，这些菌株被分为5种基因型。在从猪中分离出的菌株中，观察到携带所有三个毒力基因的基因型V占显著优势。在分离菌株中，观察到对大多数第三代和第四代头孢菌素以及环丙沙星完全敏感。值得注意的是，每个毒力基因的单独存在与对某些抗菌药物的耐药性之间存在关联，这是一个有待进一步研究的问题。
