Hulankova Radka
Department of Animal Origin Food and Gastronomic Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 612 42 Brno, Czech Republic.
Pathogens. 2022 Dec 1;11(12):1456. doi: 10.3390/pathogens11121456.
Yersiniosis is an important zoonotic disease; however, data are scarce on the resistance of enteropathogenic yersiniae, especially that of Y. pseudotuberculosis. Minimum inhibitory concentrations (MIC) of 21 antibiotics and 3 essential oils (EOs) were determined by broth microdilution for Y. enterocolitica bioserotype 4/O:3 strains isolated from domestic swine (n = 132) and Y. pseudotuberculosis strains isolated from wild boars (n = 46). For 15 of 21 antibiotics, statistically significant differences were found between MIC values of Y. enterocolitica and Y. pseudotuberculosis. While Y. enterocolitica was more resistant to amoxiclav, ampicillin, cefotaxime, cefuroxime, gentamicin, imipenem, meropenem, tetracycline, tobramycin, and trimethoprim, Y. pseudotuberculosis was more resistant to cefepime, ceftazidime, colistin, erythromycin, and nitrofurantoin. Statistically significant differences were found between various essential oils (p < 0.001) and species (p < 0.001). The lowest MICs for multiresistant Y. enterocolitica (n = 12) and Y. pseudotuberculosis (n = 12) were obtained for cinnamon (median 414 and 207 μg/mL, respectively) and oregano EOs (median 379 and 284 μg/mL), whereas thyme EO showed significantly higher MIC values (median 738 and 553 μg/mL; p < 0.001). There was no difference between Y. enterocolitica strains of plant (1A) and animal (4/O:3) origin (p = 0.855). The results show that Y. enterocolitica is generally more resistant to antimicrobials than Y. pseudotuberculosis.
耶尔森氏菌病是一种重要的人畜共患病；然而，关于肠道致病性耶尔森氏菌的耐药性，尤其是假结核耶尔森氏菌的耐药性数据却很匮乏。通过肉汤微量稀释法测定了从家猪分离的小肠结肠炎耶尔森氏菌生物血清型4/O:3菌株（n = 132）和从野猪分离的假结核耶尔森氏菌菌株（n = 46）对21种抗生素和3种精油（EOs）的最低抑菌浓度（MIC）。在21种抗生素中的15种中，小肠结肠炎耶尔森氏菌和假结核耶尔森氏菌的MIC值存在统计学显著差异。小肠结肠炎耶尔森氏菌对阿莫西林克拉维酸、氨苄西林、头孢噻肟、头孢呋辛、庆大霉素、亚胺培南、美罗培南、四环素、妥布霉素和甲氧苄啶的耐药性更强，而假结核耶尔森氏菌对头孢吡肟、头孢他啶、黏菌素、红霉素和呋喃妥因的耐药性更强。在各种精油之间（p < 0.001）和菌种之间（p < 0.001）发现了统计学显著差异。对多重耐药的小肠结肠炎耶尔森氏菌（n = 12）和假结核耶尔森氏菌（n = 12）而言，肉桂（中位数分别为414和207 μg/mL）和牛至精油（中位数分别为379和284 μg/mL）的MIC最低，而百里香精油的MIC值显著更高（中位数分别为738和553 μg/mL；p < 0.001）。植物源（1A）和动物源（4/O:3）的小肠结肠炎耶尔森氏菌菌株之间没有差异（p = 0.855）。结果表明，小肠结肠炎耶尔森氏菌通常比假结核耶尔森氏菌对抗菌药物的耐药性更强。
