Sibila M, Bernal R, Torrents D, Riera P, Llopart D, Calsamiglia M, Segalés J
Centre de Recerca en Sanitat Animal (CReSA), Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Spain.
Vet Microbiol. 2008 Feb 5;127(1-2):165-70. doi: 10.1016/j.vetmic.2007.07.027. Epub 2007 Aug 1.
The objectives of the present study were to compare Mycoplasma hyopneumoniae (Mh) colonization and serologic status on Mh vaccinated and non-vaccinated sows and to assess the effect of sow vaccination on colonization and serologic status of their piglets at weaning as well as presence of enzootic pneumonia (EP) lung lesions at slaughter. Fifty sows (25 vaccinated and 25 unvaccinated) as well as five of their piglets were included in the study. Blood samples and nasal swabs from sows at 7 weeks pre-farrowing and 1 week post-farrowing and from piglets at 3-4 weeks of age were taken. Nasal swabs and sera were tested by a nested polymerase chain reaction (nPCR) to detect Mh DNA and by an enzyme-linked immunosorbent assay (ELISA) test to detect antibodies to the pathogen, respectively. Finally, at 23 weeks of age, pigs were sent to the slaughter where the extension of EP-compatible gross lesions was assessed. Vaccination with two doses of Mh vaccine resulted in a significantly higher (p<0.05) percentage of seropositive sows than in the non-vaccinated group at 1 week post-farrowing. On the contrary, no statistical significant differences were found in the number of nasal nPCR positive sows among different treatments (p>0.05). At 3-4 weeks of age, a significantly higher percentage (p<0.001) of seropositive piglets came from vaccinated than from non-vaccinated sows. Although the number of Mh infected piglets coming from non-vaccinated sows was higher than the one from vaccinated sows, the difference was not statistically significant (p>0.05). Overall, piglets from vaccinated sows had a significant lower (p<0.05) mean of EP-compatible lung lesions (1.83+/-2.8) than piglets from non-vaccinated sows (3.02+/-3.6). Under the conditions described in this study, sow vaccination did not affect sow or piglet colonization but increased the percentage of seropositive sows and piglets at weaning and reduced significantly the mean EP-compatible lung lesion scoring at slaughter.
本研究的目的是比较猪肺炎支原体（Mh）在接种和未接种疫苗的母猪中的定植情况和血清学状态，并评估母猪接种疫苗对其仔猪断奶时的定植情况、血清学状态以及屠宰时地方性肺炎（EP）肺部病变存在情况的影响。本研究纳入了50头母猪（25头接种疫苗和25头未接种疫苗）及其5头仔猪。采集了分娩前7周和分娩后1周母猪以及3 - 4周龄仔猪的血样和鼻拭子。分别通过巢式聚合酶链反应（nPCR）检测鼻拭子和血清中的Mh DNA，并通过酶联免疫吸附测定（ELISA）检测针对该病原体的抗体。最后，在23周龄时，将猪送去屠宰，评估与EP相符的肉眼可见病变的范围。在分娩后1周，接种两剂Mh疫苗的母猪血清阳性百分比显著高于未接种疫苗组（p<0.05）。相反，不同处理组间鼻拭子nPCR阳性母猪数量无统计学显著差异（p>0.05）。在3 - 4周龄时，接种疫苗母猪的血清阳性仔猪百分比显著高于未接种疫苗母猪（p<0.001）。虽然来自未接种疫苗母猪的Mh感染仔猪数量高于接种疫苗母猪，但差异无统计学意义（p>0.05）。总体而言，接种疫苗母猪的仔猪与EP相符的肺部病变平均得分（1.83±2.8）显著低于未接种疫苗母猪的仔猪（3.02±3.6）。在本研究所述条件下，母猪接种疫苗不影响母猪或仔猪的定植情况，但增加了断奶时血清阳性母猪和仔猪的百分比，并显著降低了屠宰时与EP相符的肺部病变平均得分。
