Oğuzkurt Pelin, Kayaselçuk Fazilet, Tuncer Ilhan, Alkan Murat, Hiçsönmez Akgün
Department of Pediatric Surgery, Baskent University, Faculty of Medicine, Ankara, Turkey.
Urology. 2007 Aug;70(2):346-50. doi: 10.1016/j.urology.2007.03.030.
To investigate and compare the distribution and intensity of staining of extracellular matrix proteins--laminin, fibronectin, and types 1 and 4 collagen--in various congenital inguinoscrotal abnormalities and the peritoneum through immunohistochemical staining.
The sacs associated with undescended testis (n = 28), hydrocele (n = 29), inguinal hernia (n = 31), and parietal peritoneum (n = 28) were stained with antibodies for laminin, fibronectin, and types 1 and 4 collagen. The peritoneum served as the control group. Appropriate tissue sections of each group of samples were identified and compared with regard to the intensity and distribution of staining.
The expression of laminin was significantly greater in the sacs associated with undescended testes and inguinal hernia than in the peritoneum. The expression pattern of fibronectin did not differ in the sacs of the different inguinoscrotal abnormalities compared with peritoneum. Type 1 collagen was strongly expressed in the sacs obtained from the hydroceles compared with the other groups and the peritoneum. Expression of type 4 collagen was significantly increased in the sacs associated with hydrocele and inguinal hernia compared with the peritoneum.
The distribution and expression of laminin and types 1 and 4 collagen showed variability in the sacs associated with undescended testis, hydrocele, and inguinal hernia and the peritoneum. The tensile forces that the sacs associated with childhood inguinoscrotal abnormalities are exposed to per se could account for some of the differences seen between the inguinoscrotal tissues and anterior peritoneum. These differences in the extracellular matrix proteins could also reflect congenital properties of various childhood inguinoscrotal abnormalities.
通过免疫组织化学染色研究并比较细胞外基质蛋白——层粘连蛋白、纤连蛋白、1型和4型胶原蛋白——在各种先天性腹股沟阴囊异常及腹膜中的分布和染色强度。
用层粘连蛋白、纤连蛋白、1型和4型胶原蛋白的抗体对与隐睾（n = 28）、鞘膜积液（n = 29）、腹股沟疝（n = 31）相关的囊以及壁层腹膜（n = 28）进行染色。腹膜作为对照组。对每组样本的适当组织切片进行识别，并就染色强度和分布进行比较。
与隐睾和腹股沟疝相关的囊中，层粘连蛋白的表达明显高于腹膜。与腹膜相比，不同腹股沟阴囊异常的囊中纤连蛋白的表达模式没有差异。与其他组和腹膜相比，鞘膜积液获得的囊中1型胶原蛋白强烈表达。与腹膜相比，与鞘膜积液和腹股沟疝相关的囊中4型胶原蛋白的表达明显增加。
层粘连蛋白、1型和4型胶原蛋白在与隐睾、鞘膜积液和腹股沟疝相关的囊以及腹膜中的分布和表达存在差异。儿童腹股沟阴囊异常相关的囊所承受的拉力本身可能是腹股沟阴囊组织与前腹膜之间出现某些差异的原因。细胞外基质蛋白的这些差异也可能反映了各种儿童腹股沟阴囊异常的先天性特征。
