Wan K
Institute of Epidemiology and Microbiology, Chinese Academy of Prevetive Medicine.
Zhonghua Liu Xing Bing Xue Za Zhi. 1991 Oct;12(5):299-301.
Contaminated cultures of strains H11, H20 from ticks, and strain R9 from patient were purified by antisera for bacteria, chemicals, filtration dilution and animal inoculation. Most of bacteria were eliminated after adding antisera into contaminated strains H11, H20. The preliminarily decontaminated cultures of strains H11, H20 or those with a few bacteria such as strain R9 could be completely purified by filtration, chemicals, dilution, or animal inoculation, but filtration is the simplest and the most effective purification method.
来自蜱虫的H11、H20菌株以及来自患者的R9菌株的污染培养物,通过细菌抗血清、化学试剂、过滤稀释和动物接种进行纯化。向污染的H11、H20菌株中加入抗血清后,大部分细菌被清除。初步去污的H11、H20菌株培养物或含有少量细菌的培养物(如R9菌株)可通过过滤、化学试剂、稀释或动物接种完全纯化,但过滤是最简单且最有效的纯化方法。