Jobe D A, Callister S M, Schell R F
Microbiology Research Laboratory, Gundersen Medical Foundation, La Crosse, Wisconsin 54601.
J Clin Microbiol. 1993 Jul;31(7):1896-8. doi: 10.1128/jcm.31.7.1896-1898.1993.
Bacterial contamination frequently interferes with successful recovery of the Lyme spirochete from cultures of tissue from Borrelia burgdorferi-infected humans, rodents, or ticks. We used 0.20- and 0.45-microns-pore-size syringe-tip filters to recover spirochetes from cultures contaminated with other bacteria. Low concentrations (1 to 10/ml) of B. burgdorferi organisms could be recovered from cultures seeded with 1 x 10(8) to 4 x 10(8) Staphylococcus aureus, Streptococcus faecium, Escherichia coli, or Bacillus subtilis organisms per ml. We also used this technique to recover B. burgdorferi from contaminated environmental and clinical cultures of B. burgdorferi. We conclude the filtration is an efficient method for recovering Lyme spirochetes from contaminated samples and increasing the number of successful isolations of B. burgdorferi.
细菌污染常常干扰从感染伯氏疏螺旋体的人类、啮齿动物或蜱的组织培养物中成功复苏莱姆螺旋体。我们使用孔径为0.20微米和0.45微米的注射器针头过滤器从被其他细菌污染的培养物中回收螺旋体。每毫升接种1×10⁸至4×10⁸金黄色葡萄球菌、粪肠球菌、大肠杆菌或枯草芽孢杆菌的培养物中，可回收低浓度（1至10/毫升）的伯氏疏螺旋体。我们还使用该技术从受污染的伯氏疏螺旋体环境和临床培养物中回收伯氏疏螺旋体。我们得出结论，过滤是从受污染样本中回收莱姆螺旋体并增加伯氏疏螺旋体成功分离数量的有效方法。
