Salimi-Bejestani M R, Daniel R, Cripps P, Felstead S, Williams D J L
School of Veterinary Medicine, University of Semnan, PO Box 35195-363, Semnan, Iran.
Vet Parasitol. 2007 Nov 10;149(3-4):290-3. doi: 10.1016/j.vetpar.2007.08.008. Epub 2007 Sep 10.
Antibodies against Fasciola hepatica were detected in serum and individual milk samples of dairy cattle using an ELISA. Percentage positivity (PP) values in milk samples were related to serum PP values and were not influenced by days into lactation. The correlation coefficient between serum and individual milk samples was highly significant (r=0.84, P<0.005). The correlation coefficient between herd seroprevalence and herd milk antibody prevalence was 0.96. The correlation coefficient between prevalence measured by faecal egg count and both seroprevalence and milk antibody prevalence within the herd was 0.87. The diagnostic sensitivity and specificity for milk were 92% (95% CI=89-96) and 88% (95% CI=85-91), respectively, when the serum test was considered as a gold standard. In conclusion, the level of antibody to F. hepatica in milk is significantly correlated with the antibody level in serum and this ELISA is suitable as a means of routine veterinary diagnosis of exposure to F. hepatica in cattle and an alternative to testing sera.
使用酶联免疫吸附测定法（ELISA）在奶牛的血清和个体乳样中检测到了抗肝片吸虫抗体。乳样中的阳性百分比（PP）值与血清PP值相关，且不受泌乳天数的影响。血清与个体乳样之间的相关系数高度显著（r = 0.84，P < 0.005）。畜群血清阳性率与畜群乳抗体阳性率之间的相关系数为0.96。通过粪便虫卵计数测得的患病率与畜群内血清阳性率和乳抗体阳性率之间的相关系数为0.87。当血清检测被视为金标准时，乳的诊断敏感性和特异性分别为92%（95%置信区间 = 89 - 96）和88%（95%置信区间 = 85 - 91）。总之，乳中抗肝片吸虫抗体水平与血清中的抗体水平显著相关，并且这种ELISA适合作为牛群中肝片吸虫暴露的常规兽医诊断手段以及血清检测的替代方法。
