Mezo M, González-Warleta M, Castro-Hermida J A, Muiño L, Ubeira F M
Laboratorio de Parasitología, Centro de Investigaciones Agrarias de Mabegondo, Instituto Galego de Calidade Alimentaria-Xunta de Galicia, Abegondo, A Coruña, Spain.
Parasitol Int. 2010 Dec;59(4):610-5. doi: 10.1016/j.parint.2010.09.001. Epub 2010 Sep 15.
We carried out a field evaluation of the MM3-SERO ELISA for the diagnosis of Fasciola hepatica infection, by analysing serum and milk samples from individual cows and samples from bulk milk tanks. The diagnostic performance of the assay was assessed with serum samples from all 257 cows in eight fluke-free herds, and 240 cows with natural fasciolosis (diagnosed in vivo and/or post-mortem). Assay performance for individual milk samples was determined by analysis of paired serum and milk samples from 947 lactating cows from 33 F. hepatica-infected farms. The diagnostic usefulness of the assay for bulk tank milk was evaluated by analysis of bulk milk from infected (33) and non-infected (35) farms. For serum samples, the sensitivity, specificity and diagnostic accuracy of the assay were respectively 99.2% (95% CI: 97.0%-99.9%), 100% (95% CI: 98.6%-100%) and 0.997 (95% CI: 0.987-1.000). The only two infected animals in which serum antibodies were not detected had very low parasitic burdens (with only 2 and 3 flukes observed). The performance of the MM3 SERO ELISA for individual milk samples was similar to that for serum samples, and the stepwise linear regression revealed a strong correlation between the results for the milk samples and the serum samples (R(2)=0.84; p<0.001). The agreement between results obtained with pairs of serum and milk samples was very high: there was matching classification in 96% (910/947) of paired samples (kappa=0.92; p<0.001). Individual milk samples may therefore be used, instead of serum samples, in the MM3-SERO ELISA, for reliable detection of seropositive cows. Testing bulk tank milk samples enabled detection of infected herds, even when the within-herd prevalence of infection was as low as 12%. We conclude that the MM3-SERO ELISA is a sensitive and highly specific test for serodiagnosis of bovine fasciolosis, and can be used with individual samples of either serum or milk. Use of the assay with bulk milk samples enables estimation of the within-herd prevalence of infection.
我们通过分析个体奶牛的血清和乳样以及储奶罐中的乳样，对用于诊断肝片吸虫感染的MM3-SERO酶联免疫吸附测定法进行了现场评估。使用来自8个无吸虫牛群的所有257头奶牛的血清样本以及240头自然感染肝片吸虫的奶牛（通过活体诊断和/或死后诊断）的血清样本，评估了该测定法的诊断性能。通过分析来自33个感染肝片吸虫农场的947头泌乳奶牛的配对血清和乳样，确定了单个乳样的测定性能。通过分析感染农场（33个）和未感染农场（35个）的储奶罐乳样，评估了该测定法对储奶罐乳样的诊断效用。对于血清样本，该测定法的敏感性、特异性和诊断准确性分别为99.2%（95%置信区间：97.0%-99.9%）、100%（95%置信区间：98.6%-100%）和0.997（95%置信区间：0.987-1.000）。仅有的2头未检测到血清抗体的感染动物的寄生虫负荷非常低（仅观察到2条和3条吸虫）。MM3 SERO酶联免疫吸附测定法对单个乳样的性能与对血清样本的性能相似，逐步线性回归显示乳样和血清样本的结果之间存在强相关性（R²=0.84；p<0.001）。配对血清和乳样获得的结果之间的一致性非常高：96%（910/947）的配对样本分类匹配（kappa=0.92；p<0.001）。因此，在MM3-SERO酶联免疫吸附测定法中，可以使用单个乳样而非血清样本，以可靠地检测血清反应阳性的奶牛。检测储奶罐乳样能够检测到感染牛群，即使牛群内的感染患病率低至12%。我们得出结论，MM3-SERO酶联免疫吸附测定法是一种用于牛肝片吸虫病血清诊断的敏感且高度特异的检测方法，可用于血清或乳样的单个样本。将该测定法用于储奶罐乳样能够估计牛群内的感染患病率。
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