肉鸡坏死性肠炎发展过程中产气荚膜梭菌细胞增殖及α-毒素基因表达的定量分析
Quantification of cell proliferation and alpha-toxin gene expression of Clostridium perfringens in the development of necrotic enteritis in broiler chickens.
作者信息
Si Weiduo, Gong Joshua, Han Yanming, Yu Hai, Brennan John, Zhou Huaijun, Chen Shu
机构信息
Food Research Program, Agriculture and Agri-Food Canada, Guelph, Ontario, Canada N1G 5C9.
出版信息
Appl Environ Microbiol. 2007 Nov;73(21):7110-3. doi: 10.1128/AEM.01108-07. Epub 2007 Sep 7.
Abstract
Cell proliferation and alpha-toxin gene expression of Clostridium perfringens in relation to the development of necrotic enteritis (NE) were investigated. Unlike bacitracin-treated chickens, non-bacitracin-treated birds exhibited typical NE symptoms and reduced growth performance. They also demonstrated increased C. perfringens proliferation and alpha-toxin gene expression that were positively correlated and progressed according to the regression model y = b(0) + b(1)X - b(2)X(2). The average C. perfringens count of 5 log(10) CFU/g in the ileal digesta appears to be a threshold for developing NE with a lesion score of 2.
摘要
研究了产气荚膜梭菌的细胞增殖和α-毒素基因表达与坏死性肠炎（NE）发展的关系。与杆菌肽处理的鸡不同，未用杆菌肽处理的鸡表现出典型的NE症状，生长性能下降。它们还表现出产气荚膜梭菌增殖增加和α-毒素基因表达增加，二者呈正相关，并根据回归模型y = b(0) + b(1)X - b(2)X(2)发展。回肠食糜中产气荚膜梭菌平均计数为5 log(10) CFU/g似乎是病变评分为2的NE发生的阈值。
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