肉鸡坏死性肠炎发展过程中产气荚膜梭菌细胞增殖及α-毒素基因表达的定量分析
Quantification of cell proliferation and alpha-toxin gene expression of Clostridium perfringens in the development of necrotic enteritis in broiler chickens.
作者信息
Si Weiduo, Gong Joshua, Han Yanming, Yu Hai, Brennan John, Zhou Huaijun, Chen Shu
机构信息
Food Research Program, Agriculture and Agri-Food Canada, Guelph, Ontario, Canada N1G 5C9.
出版信息
Appl Environ Microbiol. 2007 Nov;73(21):7110-3. doi: 10.1128/AEM.01108-07. Epub 2007 Sep 7.
Abstract
Cell proliferation and alpha-toxin gene expression of Clostridium perfringens in relation to the development of necrotic enteritis (NE) were investigated. Unlike bacitracin-treated chickens, non-bacitracin-treated birds exhibited typical NE symptoms and reduced growth performance. They also demonstrated increased C. perfringens proliferation and alpha-toxin gene expression that were positively correlated and progressed according to the regression model y = b(0) + b(1)X - b(2)X(2). The average C. perfringens count of 5 log(10) CFU/g in the ileal digesta appears to be a threshold for developing NE with a lesion score of 2.
摘要
研究了产气荚膜梭菌的细胞增殖和α-毒素基因表达与坏死性肠炎（NE）发展的关系。与杆菌肽处理的鸡不同，未用杆菌肽处理的鸡表现出典型的NE症状，生长性能下降。它们还表现出产气荚膜梭菌增殖增加和α-毒素基因表达增加，二者呈正相关，并根据回归模型y = b(0) + b(1)X - b(2)X(2)发展。回肠食糜中产气荚膜梭菌平均计数为5 log(10) CFU/g似乎是病变评分为2的NE发生的阈值。
相似文献
1
Quantification of cell proliferation and alpha-toxin gene expression of Clostridium perfringens in the development of necrotic enteritis in broiler chickens.肉鸡坏死性肠炎发展过程中产气荚膜梭菌细胞增殖及α-毒素基因表达的定量分析
Appl Environ Microbiol. 2007 Nov;73(21):7110-3. doi: 10.1128/AEM.01108-07. Epub 2007 Sep 7.
2
Responses of broiler chickens orally challenged with Clostridium perfringens isolated from field cases of necrotic enteritis.用从坏死性肠炎田间病例中分离出的产气荚膜梭菌经口攻毒肉鸡的反应。
Res Vet Sci. 2006 Aug;81(1):99-108. doi: 10.1016/j.rvsc.2005.10.006. Epub 2005 Dec 9.
3
Immunization with recombinant alpha toxin partially protects broiler chicks against experimental challenge with Clostridium perfringens.用重组α毒素进行免疫可部分保护肉鸡雏鸡免受产气荚膜梭菌的实验性攻击。
Vet Microbiol. 2009 Jan 1;133(1-2):92-7. doi: 10.1016/j.vetmic.2008.06.001. Epub 2008 Jun 8.
4
Virulence for chickens of Clostridium perfringens isolated from poultry and other sources.从禽畜和其他来源分离的产气荚膜梭菌对鸡的毒力。
Anaerobe. 2010 Jun;16(3):289-92. doi: 10.1016/j.anaerobe.2010.02.006. Epub 2010 Mar 1.
5
Toxinotyping of necrotic enteritis-producing and commensal isolates of Clostridium perfringens from chickens fed organic diets.从食用有机日粮的鸡中分离出的坏死性肠炎产生菌和共生梭菌的毒素定型。
Avian Pathol. 2010 Dec;39(6):475-81. doi: 10.1080/03079457.2010.521141.
6
Immunization of broiler chickens against Clostridium perfringens-induced necrotic enteritis.用肉鸡免疫预防产气荚膜梭菌引起的坏死性肠炎。
Clin Vaccine Immunol. 2007 Sep;14(9):1070-7. doi: 10.1128/CVI.00162-07. Epub 2007 Jul 18.
7
Origin of Clostridium perfringens isolates determines the ability to induce necrotic enteritis in broilers.产气荚膜梭菌分离株的来源决定了其诱导肉鸡坏死性肠炎的能力。
Comp Immunol Microbiol Infect Dis. 2009 Nov;32(6):503-12. doi: 10.1016/j.cimid.2008.07.001. Epub 2008 Sep 9.
8
Identification of changes in the composition of ileal bacterial microbiota of broiler chickens infected with Clostridium perfringens.鉴定感染产气荚膜梭菌的肉鸡回肠细菌微生物群落组成的变化。
Vet Microbiol. 2010 Jan 6;140(1-2):116-21. doi: 10.1016/j.vetmic.2009.07.001. Epub 2009 Jul 9.
9
Necrotic enteritis-producing strains of Clostridium perfringens displace non-necrotic enteritis strains from the gut of chicks.产气荚膜梭菌的坏死性肠炎致病菌株会将非坏死性肠炎菌株从小鸡肠道中取代出来。
Vet Microbiol. 2008 Jan 25;126(4):377-82. doi: 10.1016/j.vetmic.2007.07.019. Epub 2007 Jul 25.
10
Clostridium perfringens alpha toxin is produced in the intestines of broiler chicks inoculated with an alpha toxin mutant.产气荚膜梭菌α毒素在接种α毒素突变体的肉鸡雏鸡的肠道中产生。
Anaerobe. 2010 Dec;16(6):614-7. doi: 10.1016/j.anaerobe.2010.09.006. Epub 2010 Oct 8.
引用本文的文献
1
Butyric and valeric glycerides alleviate sub-clinical necrotic enteritis effect on performance and gut health of broiler chickens.丁酸甘油酯和戊酸甘油酯可减轻亚临床坏死性肠炎对肉鸡生产性能和肠道健康的影响。
Poult Sci. 2025 Jun 13;104(9):105441. doi: 10.1016/j.psj.2025.105441.
2
Detection of necrotic enteritis risk through non-invasive monitoring of Clostridium perfringens in feces.通过对粪便中产气荚膜梭菌进行非侵入性监测来检测坏死性肠炎风险。
Poult Sci. 2025 Feb;104(2):104809. doi: 10.1016/j.psj.2025.104809. Epub 2025 Jan 13.
3
Clostridium perfringens chitinases, key enzymes during early stages of necrotic enteritis in broiler chickens.产气荚膜梭菌几丁质酶，肉鸡坏死性肠炎早期的关键酶。
PLoS Pathog. 2024 Sep 16;20(9):e1012560. doi: 10.1371/journal.ppat.1012560. eCollection 2024 Sep.
4
Investigation of Trehalose Supplementation Impacting and from Broiler Farming.海藻糖补充剂对肉鸡养殖的影响及相关研究。
Vet Sci. 2023 Jul 15;10(7):466. doi: 10.3390/vetsci10070466.
5
Probiotics mitigating subclinical necrotic enteritis (SNE) as potential alternatives to antibiotics in poultry.益生菌可缓解家禽亚临床坏死性肠炎（SNE），有望成为抗生素的替代物。
AMB Express. 2020 Mar 14;10(1):50. doi: 10.1186/s13568-020-00989-6.
6
Genetics and genomics of susceptibility and immune response to necrotic enteritis in chicken: a review.鸡坏死性肠炎易感性及免疫反应的遗传学和基因组学：综述
Mol Biol Rep. 2018 Feb;45(1):31-37. doi: 10.1007/s11033-017-4138-8. Epub 2017 Dec 20.
7
The Agr-Like Quorum Sensing System Is Required for Pathogenesis of Necrotic Enteritis Caused by Clostridium perfringens in Poultry.禽源产气荚膜梭菌引起的坏死性肠炎发病机制中需要类Agr群体感应系统。
Infect Immun. 2017 May 23;85(6). doi: 10.1128/IAI.00975-16. Print 2017 Jun.
8
In Vitro Selective Growth-Inhibitory Effect of 8-Hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a Medium Containing Chicken Ileal Digesta.8-羟基喹啉在含鸡回肠消化物培养基中对产气荚膜梭菌与双歧杆菌的体外选择性生长抑制作用
PLoS One. 2016 Dec 9;11(12):e0167638. doi: 10.1371/journal.pone.0167638. eCollection 2016.
9
Alternatives to Antibiotics to Prevent Necrotic Enteritis in Broiler Chickens: A Microbiologist's Perspective.从微生物学家角度看预防肉鸡坏死性肠炎的抗生素替代方案
Front Microbiol. 2015 Dec 1;6:1336. doi: 10.3389/fmicb.2015.01336. eCollection 2015.
10
Lactobacillus zeae protects Caenorhabditis elegans from enterotoxigenic Escherichia coli-caused death by inhibiting enterotoxin gene expression of the pathogen.玉米乳杆菌通过抑制病原性肠毒素大肠杆菌的肠毒素基因表达，保护秀丽隐杆线虫免受其致死作用。
PLoS One. 2014 Feb 18;9(2):e89004. doi: 10.1371/journal.pone.0089004. eCollection 2014.
本文引用的文献
1
Alpha-toxin of Clostridium perfringens is not an essential virulence factor in necrotic enteritis in chickens.产气荚膜梭菌的α毒素并非鸡坏死性肠炎中的必需毒力因子。
Infect Immun. 2006 Nov;74(11):6496-500. doi: 10.1128/IAI.00806-06. Epub 2006 Aug 21.
2
Evaluation of QIAamp DNA Stool Mini Kit for ecological studies of gut microbiota.用于肠道微生物群生态研究的QIAamp DNA粪便迷你试剂盒评估
J Microbiol Methods. 2003 Jul;54(1):13-20. doi: 10.1016/s0167-7012(02)00260-9.
3
The efficacy of bacitracin methylene disalicylate when fed in combination with narasin in the management of necrotic enteritis in broiler chickens.杆菌肽亚甲基二水杨酸酯与那拉菌素联合投喂对肉鸡坏死性肠炎的防治效果。
Poult Sci. 2003 Mar;82(3):360-3. doi: 10.1093/ps/82.3.360.
4
Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression.通过比较kappa和lambda免疫球蛋白轻链表达来检测B淋巴细胞单克隆性的定量实时PCR方法。
Clin Chem. 2003 Jan;49(1):51-9. doi: 10.1373/49.1.51.
5
Efficacy of in-feed tylosin phosphate for the treatment of necrotic enteritis in broiler chickens.饲料中添加磷酸泰乐菌素治疗肉鸡坏死性肠炎的效果
Poult Sci. 2001 Oct;80(10):1451-4. doi: 10.1093/ps/80.10.1451.
6
Efficacy of narasin in the prevention of necrotic enteritis in broiler chickens.甲基盐霉素预防肉鸡坏死性肠炎的效果。
Avian Dis. 2001 Jan-Mar;45(1):210-4.
7
Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers.利用变性梯度凝胶电泳和种特异性PCR引物检测与鉴定胃肠道中的乳酸杆菌种类
Appl Environ Microbiol. 2000 Jan;66(1):297-303. doi: 10.1128/AEM.66.1.297-303.2000.
8
Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens.用于产气荚膜梭菌基因分型的多重聚合酶链反应检测
Am J Vet Res. 1997 Jul;58(7):702-5.
9
Clostridial enteric diseases of domestic animals.家畜梭菌性肠道疾病
Clin Microbiol Rev. 1996 Apr;9(2):216-34. doi: 10.1128/CMR.9.2.216.
10
The occurrence of Clostridium perfringens in the intestine of chicks.
Avian Dis. 1984 Oct-Dec;28(4):1120-4.
Zotero 插件