Corbett David, Bennett Hayley J, Askar Hamdia, Green Jeffrey, Roberts Ian S
Faculty of Life Sciences, University of Manchester, Smith Bldg., Oxford Rd., Manchester M13 9PT, United Kingdom.
Faculty of Life Sciences, University of Manchester, Smith Bldg., Oxford Rd., Manchester M13 9PT, United Kingdom; Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt.
J Biol Chem. 2007 Nov 16;282(46):33326-33335. doi: 10.1074/jbc.M703465200. Epub 2007 Sep 7.
In this paper, we present the first evidence of a role for the transcriptional regulator SlyA in the regulation of transcription of the Escherichia coli K5 capsule gene cluster and demonstrate, using a combination of reporter gene fusions, DNase I footprinting, and electrophoretic mobility shift assays, the dependence of transcription on the functional interplay between H-NS and SlyA. Both SlyA and H-NS bind to multiple overlapping sites within the promoter in vitro, but their binding is not mutually exclusive, resulting in a remodeled nucleoprotein complex. In addition, we show that expression of the E. coli slyA gene is temperature-regulated, positively autoregulated, and independent of H-NS.
在本文中，我们首次证明转录调节因子SlyA在大肠杆菌K5荚膜基因簇转录调控中发挥作用，并通过报告基因融合、DNase I足迹分析和电泳迁移率变动分析相结合的方法，证明转录依赖于H-NS和SlyA之间的功能相互作用。SlyA和H-NS在体外均与启动子内多个重叠位点结合，但它们的结合并非相互排斥，从而形成重塑的核蛋白复合物。此外，我们还表明大肠杆菌slyA基因的表达受温度调节、正自调节且不依赖于H-NS。
