菜豆发育种子中两种异淀粉酶型淀粉脱支酶的链长特异性差异

Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean.

作者信息

Takashima Yoshinori, Senoura Takeshi, Yoshizaki Takayuki, Hamada Shigeki, Ito Hiroyuki, Matsui Hirokazu

机构信息

Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.

出版信息

Biosci Biotechnol Biochem. 2007 Sep;71(9):2308-12. doi: 10.1271/bbb.70215. Epub 2007 Sep 7.

DOI:10.1271/bbb.70215

PMID:17827690

Abstract

Plant isoamylase-type starch-debranching enzymes (ISAs) hydrolyze alpha-1,6-linkages in alpha-1,4/alpha-1,6-linked polyglucans. Two ISAs, designated PvISA1/2 and PvISA3, were purified from developing seeds of kidney bean by ammonium sulfate fractionation and several column chromatographic procedures. The enzymes displayed different substrate specificities for polyglucans: PvISA1/2 showed broad chain-length specificities, whereas PvISA3 liberated specific chains with a DP of 2 to 4.

摘要

植物异淀粉酶型淀粉脱支酶（ISAs）可水解α-1,4/α-1,6-连接的多聚糖中的α-1,6-糖苷键。通过硫酸铵分级分离和几种柱色谱方法，从菜豆发育中的种子中纯化出两种ISAs，分别命名为PvISA1/2和PvISA3。这些酶对多聚糖表现出不同的底物特异性：PvISA1/2具有广泛的链长特异性，而PvISA3释放出特定的2至4个葡萄糖聚合度（DP）的链。