Takahashi Masaaki, Kudaka Yuichi, Okumura Naoya, Hirano Atsushi, Banno Kazuhide, Kaneda Tsuguhiro
Department of Pharmacy, National Hospital Organization Nagoya Medical Center (Tokai Area Central Hospital for AIDS Treatment and Research), Naka-ku, Nagoya, Aichi 460-0001, Japan.
Biol Pharm Bull. 2007 Sep;30(9):1784-6. doi: 10.1248/bpb.30.1784.
The quantification of tenofovir, a nucleoside reverse transcriptase inhibitor prescribed once daily, in human plasma is important due to a recent increase in its use. HPLC, however, can not easily detect and quantify tenofovir because of interfering peaks. Therefore, we developed a rapid and conventional LC-MS method, validated by estimating the precision and accuracy for inter- and intraday analysis in the concentration range of 0.019-1.567 microg/ml. The calibration curve was linear in the described concentration range. Average accuracy ranged from 95.9 to 100.7%. Relative standard deviations of both inter- and intraday assays were less than 11.6%. Recovery of tenofovir was more than 80.2%. This novel method provides a conventional, accurate and precise way to determine tenofovir in human plasma samples.
由于近期替诺福韦（一种每日服用一次的核苷类逆转录酶抑制剂）的使用量增加，对其在人体血浆中的定量分析变得十分重要。然而，由于存在干扰峰，高效液相色谱法（HPLC）难以轻松检测和定量替诺福韦。因此，我们开发了一种快速且常规的液相色谱 - 质谱联用（LC - MS）方法，并通过在0.019 - 1.567微克/毫升的浓度范围内评估日间和日内分析的精密度与准确度进行了验证。在所描述的浓度范围内，校准曲线呈线性。平均准确度在95.9%至100.7%之间。日间和日内测定的相对标准偏差均小于11.6%。替诺福韦的回收率超过80.2%。这种新方法为测定人体血浆样本中的替诺福韦提供了一种常规、准确且精确的方式。
