使用填充有聚乙烯聚吡咯烷酮（PVPP）的小柱改进用于测定茶叶中咖啡因的样品预处理方法。

Improved sample pre-treatment for determination of caffeine in tea using a cartridge filled with polyvinylpolypyrroridone (PVPP).

作者信息

Yamauchi Yuji, Nakamura Akiko, Kitai Miki, Hatanaka Kirara, Kohno Iho, Tanimoto Tsuyoshi

机构信息

Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kyoto, Japan.

出版信息

Chem Pharm Bull (Tokyo). 2007 Sep;55(9):1393-6. doi: 10.1248/cpb.55.1393.

DOI:10.1248/cpb.55.1393

PMID:17827770

Abstract

We have improved sample pre-treatment for the effective removal of polyphenols and simple analysis of caffeine in tea using a cartridge filled with polyvinylpolypyrroridone (PVPP). Nearly 100% of catechins were removed from the green tea sample and caffeine was completely recovered in the range of 98.2-101.3% by sample pre-treatment with a PVPP cartridge. Reproducibility of preparing PVPP pre-treatment cartridges was sufficient for quantitative analysis, because RSDs of analytical values for caffeine obtained by using three individual pre-treatment cartridges filled with 10-200 mg PVPP were 0.60-2.8%. The PVPP pre-treatment cartridge also removed polyphenols perfectly and recovered caffeine faultlessly from oolong and black tea samples. Comparison with the conventional method without sample pre-treatment indicated that the present pre-treatment method with a PVPP cartridge was useful for the simple and precise analysis of caffeine in green, oolong and black tea samples.

摘要

我们改进了样品预处理方法，使用填充有聚乙烯聚吡咯烷酮（PVPP）的小柱有效去除茶叶中的多酚并对咖啡因进行简单分析。通过用PVPP小柱进行样品预处理，绿茶样品中近100%的儿茶素被去除，咖啡因的回收率在98.2 - 101.3%之间，完全回收。制备PVPP预处理小柱的重现性足以进行定量分析，因为使用三个填充10 - 200 mg PVPP的单独预处理小柱获得的咖啡因分析值的相对标准偏差（RSD）为0.60 - 2.8%。PVPP预处理小柱也能完美去除多酚，并从乌龙茶和红茶样品中完美回收咖啡因。与未进行样品预处理的传统方法相比，表明目前使用PVPP小柱的预处理方法对于绿茶、乌龙茶和红茶样品中咖啡因的简单精确分析很有用。