金属硫蛋白对离体大鼠心脏的影响。

Effects of metallothionein on isolated rat heart.

作者信息

Sun Zhongdong, Xia Jiahong, Dong Nianguo, Du Xinling, Chi Yifan, Yang Tienan, Yang Chenyuan

机构信息

Department of Cardiovascular Surgery, Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao 266011, China.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2007 Aug;27(4):448-50. doi: 10.1007/s11596-007-0425-2.

DOI:10.1007/s11596-007-0425-2

PMID:17828508

Abstract

To investigate the effects of metallothionein (MT) on isolated rat heart, 16 Wistar rats were randomly divided into 2 groups. In control group (group C), distilled water was injected intraperitoneally and 24 h later isolated hearts were perfused with Langendorff and stored at 4 degrees C for 3 h with histidine-tryptophan-ketoglutarate (HTK) solutions, and then isolated hearts were perfused for 2 h by Langendorff. In experimental group (group E), 3.6% ZnSO(4) was injected intraperitoneally, 24 h later isolated hearts were perfused by Langendorff and stored at 4 degrees C for 3 h with HTK solutions, and then the isolated hearts were perfused for 2 h with Langendorff. MT content, the recovery of hemodynamics, myocardial water content (MWC), lactate dehydrogenase (LDH) and creatine kinase (CK) leakage, adenosine triphosphate (ATP) and malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, myocardial cell Ca(2+) content, Ca(2+)-ATPase activity of mitochondria (Ca(2+)-ATPase) and its Ca(2+) content (Ca(2+)), synthesizing ATP activity of mitochondria (ATP), and the ultrastructure of cells were examined. There were a significant increase in group E in hemodynamic recovery, ATP content, SOD activity, Ca(2+)-ATPase activity, ATP activity, and substantial reduction in MWC, LDH and CK leakage, MDA content, myocardial cell Ca(2+) content, Ca(2+) content, and the ultrastructural injury were obviously milder than that of group C. This study demonstrated that MT has protective effects on isolated rat heart.

摘要

为研究金属硫蛋白（MT）对离体大鼠心脏的影响，将16只Wistar大鼠随机分为2组。对照组（C组）腹腔注射蒸馏水，24小时后用Langendorff法灌注离体心脏，并用组氨酸 - 色氨酸 - 酮戊二酸（HTK）溶液在4℃保存3小时，然后再用Langendorff法灌注2小时。实验组（E组）腹腔注射3.6%硫酸锌，24小时后用Langendorff法灌注离体心脏，并用HTK溶液在4℃保存3小时，然后再用Langendorff法灌注2小时。检测MT含量、血流动力学恢复情况、心肌含水量（MWC）、乳酸脱氢酶（LDH）和肌酸激酶（CK）漏出量、三磷酸腺苷（ATP）和丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性、心肌细胞Ca(2+)含量、线粒体Ca(2+)-ATP酶活性（Ca(2+)-ATPase）及其Ca(2+)含量（Ca(2+)）、线粒体合成ATP活性（ATP）以及细胞超微结构。结果显示，E组血流动力学恢复、ATP含量、SOD活性、Ca(2+)-ATPase活性、ATP活性显著增加，MWC、LDH和CK漏出量、MDA含量、心肌细胞Ca(2+)含量、Ca(2+)含量显著降低，且超微结构损伤明显轻于C组。本研究表明MT对离体大鼠心脏具有保护作用。